Isolation and characterization of pirital virus, a newly discovered south American arenavirus

Charles F. Fulhorst, Michael D. Bowen, Rosa Alba Salas, Nuris M C De Manzione, Gloria Duno, Antonio Utrera, Thomas Ksiazek, C. J. Peters, Stuart T. Nichol, Edith De Miller, Duilia Tovar, Bricio Ramos, Clovis Vasquez, Robert B. Tesh

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Specific rodent species are principal hosts for each of the well- characterized members of the virus family Arenaviridae. Guanarito virus (Arenaviridae) is the etiologic agent of Venezuelan hemorrhagic fever. A previous study on the epidemiology of Venezuelan hemorrhagic fever revealed extensive arenavirus infection (presumed to be caused by Guanarito virus) in two rodent species, Sigmodon alstoni and Zygodontomys brevicauda, collected from the region of Venezuela in which the disease is endemic. In the present study, four arenavirus isolates recovered from the Municipality of Guanarito (two isolates each from S. alstoni and Z. brevicauda) were characterized to learn more about the natural rodent host relationships of Guanarito virus. Serologic tests and analyses of nucleocapsid protein gene sequence data indicated that the two isolates from Z. brevicauda are strains of Guanarito virus and that the two isolates from S. alstoni are representatives of a novel New World arenavirus (proposed name Pirital) that is antigenically and phylogenetically distinct from all known New World arenaviruses. The results of the present study provide further evidence that the cane mouse Z. brevicauda is a natural host of Guanarito virus and suggest that the cotton rat S. alstoni is the natural reservoir host of Pirital but not Guanarito virus.

Original languageEnglish (US)
Pages (from-to)548-553
Number of pages6
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume56
Issue number5
StatePublished - May 1997
Externally publishedYes

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New World Arenaviruses
Arenavirus
Arenaviridae
Sigmodontinae
Rodentia
Arenaviridae Infections
Fever
Nucleocapsid Proteins
Endemic Diseases
Canes
Venezuela
Serologic Tests
Names
Epidemiology
Viruses

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

Fulhorst, C. F., Bowen, M. D., Salas, R. A., De Manzione, N. M. C., Duno, G., Utrera, A., ... Tesh, R. B. (1997). Isolation and characterization of pirital virus, a newly discovered south American arenavirus. American Journal of Tropical Medicine and Hygiene, 56(5), 548-553.

Isolation and characterization of pirital virus, a newly discovered south American arenavirus. / Fulhorst, Charles F.; Bowen, Michael D.; Salas, Rosa Alba; De Manzione, Nuris M C; Duno, Gloria; Utrera, Antonio; Ksiazek, Thomas; Peters, C. J.; Nichol, Stuart T.; De Miller, Edith; Tovar, Duilia; Ramos, Bricio; Vasquez, Clovis; Tesh, Robert B.

In: American Journal of Tropical Medicine and Hygiene, Vol. 56, No. 5, 05.1997, p. 548-553.

Research output: Contribution to journalArticle

Fulhorst, CF, Bowen, MD, Salas, RA, De Manzione, NMC, Duno, G, Utrera, A, Ksiazek, T, Peters, CJ, Nichol, ST, De Miller, E, Tovar, D, Ramos, B, Vasquez, C & Tesh, RB 1997, 'Isolation and characterization of pirital virus, a newly discovered south American arenavirus', American Journal of Tropical Medicine and Hygiene, vol. 56, no. 5, pp. 548-553.
Fulhorst CF, Bowen MD, Salas RA, De Manzione NMC, Duno G, Utrera A et al. Isolation and characterization of pirital virus, a newly discovered south American arenavirus. American Journal of Tropical Medicine and Hygiene. 1997 May;56(5):548-553.
Fulhorst, Charles F. ; Bowen, Michael D. ; Salas, Rosa Alba ; De Manzione, Nuris M C ; Duno, Gloria ; Utrera, Antonio ; Ksiazek, Thomas ; Peters, C. J. ; Nichol, Stuart T. ; De Miller, Edith ; Tovar, Duilia ; Ramos, Bricio ; Vasquez, Clovis ; Tesh, Robert B. / Isolation and characterization of pirital virus, a newly discovered south American arenavirus. In: American Journal of Tropical Medicine and Hygiene. 1997 ; Vol. 56, No. 5. pp. 548-553.
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