Isolation and characterization of the RAD2 gene of Saccharomyces cerevisiae

David R. Higgins; Louise Prakash; Paul Reynolds; Satya Prakash

doi:10.1016/0378-1119(84)90112-4

Isolation and characterization of the RAD2 gene of Saccharomyces cerevisiae

David R. Higgins, Louise Prakash, Paul Reynolds, Satya Prakash

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

We have cloned the RAD2 gene of Saccharomyces cerevisiae and used it to determine the size and direction of its transcript and to make rad2 deletion mutants. The RAD2 gene encodes a 3.3-kb transcript and the direction of transcription is leftwards, from EcoRI towards BglII. Deletions of the RAD2 gene have no effect on viability of vegetative cells or spores, or on sporulation.

Original language	English (US)
Pages (from-to)	121-128
Number of pages	8
Journal	Gene
Volume	30
Issue number	1-3
DOIs	https://doi.org/10.1016/0378-1119(84)90112-4
State	Published - Oct 1984
Externally published	Yes

Keywords

DNA repair
Escherichia coli
UV sensitivity
deletion mutants
excision
incision
pyrimidine dimers
yeast

ASJC Scopus subject areas

Genetics

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10.1016/0378-1119(84)90112-4

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abstract = "We have cloned the RAD2 gene of Saccharomyces cerevisiae and used it to determine the size and direction of its transcript and to make rad2 deletion mutants. The RAD2 gene encodes a 3.3-kb transcript and the direction of transcription is leftwards, from EcoRI towards BglII. Deletions of the RAD2 gene have no effect on viability of vegetative cells or spores, or on sporulation.",

keywords = "DNA repair, Escherichia coli, UV sensitivity, deletion mutants, excision, incision, pyrimidine dimers, yeast",

author = "Higgins, {David R.} and Louise Prakash and Paul Reynolds and Satya Prakash",

year = "1984",

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doi = "10.1016/0378-1119(84)90112-4",

language = "English (US)",

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pages = "121--128",

journal = "Gene",

issn = "0378-1119",

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TY - JOUR

T1 - Isolation and characterization of the RAD2 gene of Saccharomyces cerevisiae

AU - Higgins, David R.

AU - Prakash, Louise

AU - Reynolds, Paul

AU - Prakash, Satya

PY - 1984/10

Y1 - 1984/10

N2 - We have cloned the RAD2 gene of Saccharomyces cerevisiae and used it to determine the size and direction of its transcript and to make rad2 deletion mutants. The RAD2 gene encodes a 3.3-kb transcript and the direction of transcription is leftwards, from EcoRI towards BglII. Deletions of the RAD2 gene have no effect on viability of vegetative cells or spores, or on sporulation.

AB - We have cloned the RAD2 gene of Saccharomyces cerevisiae and used it to determine the size and direction of its transcript and to make rad2 deletion mutants. The RAD2 gene encodes a 3.3-kb transcript and the direction of transcription is leftwards, from EcoRI towards BglII. Deletions of the RAD2 gene have no effect on viability of vegetative cells or spores, or on sporulation.

KW - DNA repair

KW - Escherichia coli

KW - UV sensitivity

KW - deletion mutants

KW - excision

KW - incision

KW - pyrimidine dimers

KW - yeast

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DO - 10.1016/0378-1119(84)90112-4

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AN - SCOPUS:0021716869

SN - 0378-1119

VL - 30

SP - 121

EP - 128

JO - Gene

JF - Gene

IS - 1-3

ER -

Isolation and characterization of the RAD2 gene of Saccharomyces cerevisiae

Abstract

Keywords

ASJC Scopus subject areas

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