Isolation and characterization of the RAD2 gene of Saccharomyces cerevisiae

David R. Higgins, Louise Prakash, Paul Reynolds, Satya Prakash

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

We have cloned the RAD2 gene of Saccharomyces cerevisiae and used it to determine the size and direction of its transcript and to make rad2 deletion mutants. The RAD2 gene encodes a 3.3-kb transcript and the direction of transcription is leftwards, from EcoRI towards BglII. Deletions of the RAD2 gene have no effect on viability of vegetative cells or spores, or on sporulation.

Original languageEnglish (US)
Pages (from-to)121-128
Number of pages8
JournalGene
Volume30
Issue number1-3
DOIs
StatePublished - 1984
Externally publishedYes

Fingerprint

Saccharomyces cerevisiae
Gene Deletion
Spores
Genes
Cell Survival
Direction compound

Keywords

  • deletion mutants
  • DNA repair
  • Escherichia coli
  • excision
  • incision
  • pyrimidine dimers
  • UV sensitivity
  • yeast

ASJC Scopus subject areas

  • Genetics

Cite this

Isolation and characterization of the RAD2 gene of Saccharomyces cerevisiae. / Higgins, David R.; Prakash, Louise; Reynolds, Paul; Prakash, Satya.

In: Gene, Vol. 30, No. 1-3, 1984, p. 121-128.

Research output: Contribution to journalArticle

Higgins, David R. ; Prakash, Louise ; Reynolds, Paul ; Prakash, Satya. / Isolation and characterization of the RAD2 gene of Saccharomyces cerevisiae. In: Gene. 1984 ; Vol. 30, No. 1-3. pp. 121-128.
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