Isolation and characterization of the RAD2 gene of Saccharomyces cerevisiae

David R. Higgins, Louise Prakash, Paul Reynolds, Satya Prakash

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

We have cloned the RAD2 gene of Saccharomyces cerevisiae and used it to determine the size and direction of its transcript and to make rad2 deletion mutants. The RAD2 gene encodes a 3.3-kb transcript and the direction of transcription is leftwards, from EcoRI towards BglII. Deletions of the RAD2 gene have no effect on viability of vegetative cells or spores, or on sporulation.

Original languageEnglish (US)
Pages (from-to)121-128
Number of pages8
JournalGene
Volume30
Issue number1-3
DOIs
StatePublished - Oct 1984
Externally publishedYes

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Keywords

  • DNA repair
  • Escherichia coli
  • UV sensitivity
  • deletion mutants
  • excision
  • incision
  • pyrimidine dimers
  • yeast

ASJC Scopus subject areas

  • Genetics

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