Isolation and characterization of Whitewater Arroyo virus, a novel North American arenavirus

Charles F. Fulhorst, Michael D. Bowen, Thomas Ksiazek, Pierre E. Rollin, Stuart T. Nichol, Michael Y. Kosoy, Clarence J. Peters

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

Rodents are principal hosts for each of the well-characterized arenaviruses. Prior to the present study, Tamiami (TAM) virus was the sole arenavirus known to be indigenous to North America; it has been isolated only from southern Florida where its primary host is the cotton rat Sigmodonon hispidus. Recently, arenavirus antibody was found in Neotoma albigula woodrats collected from the southwestern United States. The purpose of the present study was to isolate and characterize the arenavirus associated with N. albigula. Three isolates of a novel arenavirus (proposed name 'Whitewater Arroyo,' WWA) were recovered from two arenavirus antibody-positive N. albigula collected from Whitewater Arroyo in McKinley County, New Mexico. Two-way serologic tests indicated that WWA virus is antigenically distinct from other arenaviruses but most closely related to TAM virus. Phylogenetic analysis of nucleocapsid protein gene sequence data showed that WWA virus is a novel arenavirus that is genetically most closely related to TAM virus. The recovery of WWA virus from antibody-positive N. albigula suggests that WWA virus infection in this species can be chronic and thus that N. albigula is a reservoir host of the virus.

Original languageEnglish (US)
Pages (from-to)114-120
Number of pages7
JournalVirology
Volume224
Issue number1
DOIs
StatePublished - Oct 1 1996
Externally publishedYes

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Arenavirus
Viruses
Sigmodontinae
Antibodies
Southwestern United States
Nucleocapsid Proteins
Serologic Tests
Virus Diseases
North America
Names
Rodentia

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Fulhorst, C. F., Bowen, M. D., Ksiazek, T., Rollin, P. E., Nichol, S. T., Kosoy, M. Y., & Peters, C. J. (1996). Isolation and characterization of Whitewater Arroyo virus, a novel North American arenavirus. Virology, 224(1), 114-120. https://doi.org/10.1006/viro.1996.0512

Isolation and characterization of Whitewater Arroyo virus, a novel North American arenavirus. / Fulhorst, Charles F.; Bowen, Michael D.; Ksiazek, Thomas; Rollin, Pierre E.; Nichol, Stuart T.; Kosoy, Michael Y.; Peters, Clarence J.

In: Virology, Vol. 224, No. 1, 01.10.1996, p. 114-120.

Research output: Contribution to journalArticle

Fulhorst, CF, Bowen, MD, Ksiazek, T, Rollin, PE, Nichol, ST, Kosoy, MY & Peters, CJ 1996, 'Isolation and characterization of Whitewater Arroyo virus, a novel North American arenavirus', Virology, vol. 224, no. 1, pp. 114-120. https://doi.org/10.1006/viro.1996.0512
Fulhorst, Charles F. ; Bowen, Michael D. ; Ksiazek, Thomas ; Rollin, Pierre E. ; Nichol, Stuart T. ; Kosoy, Michael Y. ; Peters, Clarence J. / Isolation and characterization of Whitewater Arroyo virus, a novel North American arenavirus. In: Virology. 1996 ; Vol. 224, No. 1. pp. 114-120.
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