Isolation and culture of brain microvascular endothelial cells for in vitro blood-brain barrier studies

Hector Rosas-Hernandez, Elvis Cuevas, Susan M. Lantz, Merle G. Paule, Syed F. Ali

Research output: Chapter in Book/Report/Conference proceedingChapter

16 Scopus citations

Abstract

The blood-brain barrier (BBB) is essential to maintain the proper microenvironment for brain function. Although formed by different cell types, the endothelial cells (ECs) of the brain microvessels provide the BBB with its selective permeability. To study the BBB in vitro, EC lines as well as primary isolated ECs have been used. In this chapter, we will provide a detailed protocol on how to isolate and culture primary brain microvascular endothelial cells from different species for use as in vitro models of the BBB. When performed properly, this protocol will allow one to obtain a pure culture of brain microvascular endothelial cells with which to analyze the effects of therapeutic and toxic agents on BBB functions.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages315-331
Number of pages17
DOIs
StatePublished - 2018
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1727
ISSN (Print)1064-3745

Keywords

  • Blood-brain barrier
  • Brain microvascular endothelial cells
  • Permeability
  • Trans-endothelial electrical resistance

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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