@inbook{c6ced392a7ea4d48914591d11b243768,
title = "Isolation and culture of brain microvascular endothelial cells for in vitro blood-brain barrier studies",
abstract = "The blood-brain barrier (BBB) is essential to maintain the proper microenvironment for brain function. Although formed by different cell types, the endothelial cells (ECs) of the brain microvessels provide the BBB with its selective permeability. To study the BBB in vitro, EC lines as well as primary isolated ECs have been used. In this chapter, we will provide a detailed protocol on how to isolate and culture primary brain microvascular endothelial cells from different species for use as in vitro models of the BBB. When performed properly, this protocol will allow one to obtain a pure culture of brain microvascular endothelial cells with which to analyze the effects of therapeutic and toxic agents on BBB functions.",
keywords = "Blood-brain barrier, Brain microvascular endothelial cells, Permeability, Trans-endothelial electrical resistance",
author = "Hector Rosas-Hernandez and Elvis Cuevas and Lantz, {Susan M.} and Paule, {Merle G.} and Ali, {Syed F.}",
note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media, LLC 2018.",
year = "2018",
doi = "10.1007/978-1-4939-7571-6_23",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "315--331",
booktitle = "Methods in Molecular Biology",
}