Isolation and Genome Phylogenetic Analysis of Arthropod-Borne Viruses, Including Akabane Virus, from Mosquitoes Collected in Hunan Province, China

Yuxi Cao, Shihong Fu, Song Song, Liang Cai, Hong Zhang, Lidong Gao, Lei Cao, Minghua Li, Xiaoyan Gao, Ying He, Huanyu Wang, Guodong Liang

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

This study investigated the abundance of mosquitoes and circulation of mosquito-borne arboviruses from 16 villages in 8 cities of Hunan Province, China, in July-August of 2010 and in August of 2011. In total, 16,076 mosquitoes consisting of seven species from four genera were collected by ultraviolet-light trap. Culex quinquefasciatus was the most common species, accounting for 50.63% (8140/16,076) of the total. Anopheles sinensis (24.26%, 3900/16,076) made up the second most common species, followed by Culex tritaeniorhynchus (9.76%, 1569/16,076). The proportions of Culex pipiens pallens, Armigeres subalbatus, and Culex modestus were 6.7%, 5.2%, and 3.31%, respectively. Fourteen Aedes albopictus were detected. The mosquitoes were identified by morphologic characteristics and frozen in liquid nitrogen. The mosquitoes were pooled, triturated, and centrifuged. The clarified supernatant was used to inoculate monolayers of C6/36 and baby hamster kidney-21 cells. We obtained six virus isolates that caused cytopathic effects. Phylogenetic analysis revealed that two isolates were Akabane virus (AKAV, from A. sinensis and C. quinquefasciatus), two isolates were Japanese encephalitis virus (from C. pipiens pallens and C. quinquefasciatus), and two isolates were Tibet orbivirus (from C. quinquefasciatus and C. tritaeniorhynchus). This is the first report of AKAV isolated from A. sinensis and C. quinquefasciatus in nature in China. The detection of AKAV in these species confirms circulation of AKAV in Hunan province and suggests potential challenges to the prevention and control of arthropod-borne animal viruses in mainland China.

Original languageEnglish (US)
Pages (from-to)62-72
Number of pages11
JournalVector-Borne and Zoonotic Diseases
Volume19
Issue number1
DOIs
StatePublished - Jan 2019
Externally publishedYes

Keywords

  • Akabane virus
  • Arbovirus surveillance
  • Japanese encephalitis virus
  • Mosquito-borne virus
  • Tibet orbivirus

ASJC Scopus subject areas

  • Microbiology
  • Infectious Diseases
  • Virology

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