Isolation and identification of HN-98 strain of spotted fever group rickettsiae

B. Chang; J. Zhang; X. Tian; D. Bi; J. Cai; Y. Zhang; Z. Lu

Isolation and identification of HN-98 strain of spotted fever group rickettsiae

B. Chang, J. Zhang, X. Tian, D. Bi, J. Cai, Y. Zhang, Z. Lu

Research output: Contribution to journal › Article › peer-review

Abstract

OBJECTIVE: One strain of rickettsiae was isolated from a patient with unknown fever from Qiongzhong county in Hainan Province in 1998, using embryonated hen eggs and proved to be the member of rickettsiae by the methods of morphology. It was named HS-98 strain after the name of the place and the year it was isolated. METHODS: The isolate was identified by methods of Microcomplement fixation assay and OCR/RFLP and compared with known species and strains of SFGR by DNA polymerase chain reaction and DNA polymerase chainreaction and was followed by restriction endonuclease fragment length polymorphism analysis. RESULTS: Results demonstrated that HN-98 strain was antigenically and genotypically identical to R. sibirica 246. CONCLUSION: The results suggested that there existed natural foci of spotted fever in Qiongzhong Area of Hainan Province.

Original language	English (US)
Pages (from-to)	202-204
Number of pages	3
Journal	Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi
Volume	21
Issue number	3
State	Published - Jun 2000
Externally published	Yes

ASJC Scopus subject areas

General Medicine

Cite this

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title = "Isolation and identification of HN-98 strain of spotted fever group rickettsiae",

abstract = "OBJECTIVE: One strain of rickettsiae was isolated from a patient with unknown fever from Qiongzhong county in Hainan Province in 1998, using embryonated hen eggs and proved to be the member of rickettsiae by the methods of morphology. It was named HS-98 strain after the name of the place and the year it was isolated. METHODS: The isolate was identified by methods of Microcomplement fixation assay and OCR/RFLP and compared with known species and strains of SFGR by DNA polymerase chain reaction and DNA polymerase chainreaction and was followed by restriction endonuclease fragment length polymorphism analysis. RESULTS: Results demonstrated that HN-98 strain was antigenically and genotypically identical to R. sibirica 246. CONCLUSION: The results suggested that there existed natural foci of spotted fever in Qiongzhong Area of Hainan Province.",

author = "B. Chang and J. Zhang and X. Tian and D. Bi and J. Cai and Y. Zhang and Z. Lu",

year = "2000",

month = jun,

language = "English (US)",

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journal = "Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi",

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publisher = "Zhonghua Yixuehui Zazhishe",

number = "3",

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TY - JOUR

T1 - Isolation and identification of HN-98 strain of spotted fever group rickettsiae

AU - Chang, B.

AU - Zhang, J.

AU - Tian, X.

AU - Bi, D.

AU - Cai, J.

AU - Zhang, Y.

AU - Lu, Z.

PY - 2000/6

Y1 - 2000/6

N2 - OBJECTIVE: One strain of rickettsiae was isolated from a patient with unknown fever from Qiongzhong county in Hainan Province in 1998, using embryonated hen eggs and proved to be the member of rickettsiae by the methods of morphology. It was named HS-98 strain after the name of the place and the year it was isolated. METHODS: The isolate was identified by methods of Microcomplement fixation assay and OCR/RFLP and compared with known species and strains of SFGR by DNA polymerase chain reaction and DNA polymerase chainreaction and was followed by restriction endonuclease fragment length polymorphism analysis. RESULTS: Results demonstrated that HN-98 strain was antigenically and genotypically identical to R. sibirica 246. CONCLUSION: The results suggested that there existed natural foci of spotted fever in Qiongzhong Area of Hainan Province.

AB - OBJECTIVE: One strain of rickettsiae was isolated from a patient with unknown fever from Qiongzhong county in Hainan Province in 1998, using embryonated hen eggs and proved to be the member of rickettsiae by the methods of morphology. It was named HS-98 strain after the name of the place and the year it was isolated. METHODS: The isolate was identified by methods of Microcomplement fixation assay and OCR/RFLP and compared with known species and strains of SFGR by DNA polymerase chain reaction and DNA polymerase chainreaction and was followed by restriction endonuclease fragment length polymorphism analysis. RESULTS: Results demonstrated that HN-98 strain was antigenically and genotypically identical to R. sibirica 246. CONCLUSION: The results suggested that there existed natural foci of spotted fever in Qiongzhong Area of Hainan Province.

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JO - Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi

JF - Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi

IS - 3

ER -

Isolation and identification of HN-98 strain of spotted fever group rickettsiae

Abstract

ASJC Scopus subject areas

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