Isolation and identification of spotted fever group Rickettsiae BJ-93 strain

Jianzhi Zhang, Mingyuan Fan, Dezeng Bi

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

One strain of rickettsiae was isolated from Dermacentor sinicus collected from Changping county in Beijing city in 1993 by using embryonated hen's eggs and proved to be the member of spotted fever group(SFG)rickettsiae. It was named as BJ-93 after the name of the place and the year which it was isolated. The isolate was compared with known species and strains of SFGR by micro-immunofluorescence assay, DNA polymerase chain reaction, DNA polymerase chain reaction followed by restriction endonuclease fragment length polymorphism analysis, sodium dodecylsulfate-polyacrylamide gel electrophoresis, protein immunoblotting with polyclonal and monoclonal antibodies, dot immunobinding assay. The results demonstrated that BJ-93 strain was antigenically and genotypically identical to BJ-90 strain, and genotypically identical and antigenically related to R. sibirica 246 strain but its profile was different.

Original languageEnglish (US)
Pages (from-to)28-33
Number of pages6
JournalChinese Journal of Microbiology and Immunology
Volume17
Issue number1
StatePublished - Jan 1997
Externally publishedYes

Fingerprint

Rickettsia
DNA-Directed DNA Polymerase
Fever
Dermacentor
Polymerase Chain Reaction
DNA Restriction Enzymes
Immunoblotting
Restriction Fragment Length Polymorphisms
Eggs
Names
Fluorescent Antibody Technique
Polyacrylamide Gel Electrophoresis
Sodium
Monoclonal Antibodies
Proteins
Beijing

Keywords

  • Abtibodijes, monoclonal
  • Immunoblotting
  • Immunoenzyme techqniques
  • Polymerase chain reaction
  • Spotted fever group rickettsiae

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Microbiology (medical)

Cite this

Isolation and identification of spotted fever group Rickettsiae BJ-93 strain. / Zhang, Jianzhi; Fan, Mingyuan; Bi, Dezeng.

In: Chinese Journal of Microbiology and Immunology, Vol. 17, No. 1, 01.1997, p. 28-33.

Research output: Contribution to journalArticle

Zhang, Jianzhi ; Fan, Mingyuan ; Bi, Dezeng. / Isolation and identification of spotted fever group Rickettsiae BJ-93 strain. In: Chinese Journal of Microbiology and Immunology. 1997 ; Vol. 17, No. 1. pp. 28-33.
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