Isolation and quantitative analysis of hydroxylysine glycosides

R. M. Napoli, B. S. Middleditch, N. M. Cintron, Y. M. Chen

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Methods are described for the isolation of galactosyl hydroxylysine and glucosylgalactosyl hydroxylysine from collagen and for the quantitative analysis of these hydroxylysine glycosides. The isolation procedure, based upon gel filtration and preparative ionpaired reversed-phase high-performance liquid chromatography, is simple and rapid in comparison with existing methods, allowing the production of tens of milligrams of each glycoside in a single day. Quantitative analysis of the hydroxylysine glycosides is effected by reversed-phase high-performance liquid chromatography with precolumn derivatization (using o-phthal-dialdehyde) and fluorescence detection.

Original languageEnglish (US)
Pages (from-to)497-501
Number of pages5
JournalChromatographia
Volume28
Issue number9-10
DOIs
StatePublished - Nov 1 1989
Externally publishedYes

Keywords

  • Amino acid derivatives
  • Collagen metabolites
  • Column liquid chromatography
  • Glycosides

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Organic Chemistry

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