Isolation and quantitative analysis of hydroxylysine glycosides

R. M. Napoli, B. S. Middleditch, Nitza Cintron, Y. M. Chen

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Methods are described for the isolation of galactosyl hydroxylysine and glucosylgalactosyl hydroxylysine from collagen and for the quantitative analysis of these hydroxylysine glycosides. The isolation procedure, based upon gel filtration and preparative ionpaired reversed-phase high-performance liquid chromatography, is simple and rapid in comparison with existing methods, allowing the production of tens of milligrams of each glycoside in a single day. Quantitative analysis of the hydroxylysine glycosides is effected by reversed-phase high-performance liquid chromatography with precolumn derivatization (using o-phthal-dialdehyde) and fluorescence detection.

Original languageEnglish (US)
Pages (from-to)497-501
Number of pages5
JournalChromatographia
Volume28
Issue number9-10
DOIs
StatePublished - Nov 1989
Externally publishedYes

Fingerprint

Hydroxylysine
High performance liquid chromatography
Reverse-Phase Chromatography
Glycosides
Chemical analysis
High Pressure Liquid Chromatography
Collagen
Gels
Fluorescence
Gel Chromatography
hydroxylysine glycoside

Keywords

  • Amino acid derivatives
  • Collagen metabolites
  • Column liquid chromatography
  • Glycosides

ASJC Scopus subject areas

  • Analytical Chemistry
  • Clinical Biochemistry

Cite this

Isolation and quantitative analysis of hydroxylysine glycosides. / Napoli, R. M.; Middleditch, B. S.; Cintron, Nitza; Chen, Y. M.

In: Chromatographia, Vol. 28, No. 9-10, 11.1989, p. 497-501.

Research output: Contribution to journalArticle

Napoli, RM, Middleditch, BS, Cintron, N & Chen, YM 1989, 'Isolation and quantitative analysis of hydroxylysine glycosides', Chromatographia, vol. 28, no. 9-10, pp. 497-501. https://doi.org/10.1007/BF02261068
Napoli, R. M. ; Middleditch, B. S. ; Cintron, Nitza ; Chen, Y. M. / Isolation and quantitative analysis of hydroxylysine glycosides. In: Chromatographia. 1989 ; Vol. 28, No. 9-10. pp. 497-501.
@article{818c725c958f4f399d6966d9d5257966,
title = "Isolation and quantitative analysis of hydroxylysine glycosides",
abstract = "Methods are described for the isolation of galactosyl hydroxylysine and glucosylgalactosyl hydroxylysine from collagen and for the quantitative analysis of these hydroxylysine glycosides. The isolation procedure, based upon gel filtration and preparative ionpaired reversed-phase high-performance liquid chromatography, is simple and rapid in comparison with existing methods, allowing the production of tens of milligrams of each glycoside in a single day. Quantitative analysis of the hydroxylysine glycosides is effected by reversed-phase high-performance liquid chromatography with precolumn derivatization (using o-phthal-dialdehyde) and fluorescence detection.",
keywords = "Amino acid derivatives, Collagen metabolites, Column liquid chromatography, Glycosides",
author = "Napoli, {R. M.} and Middleditch, {B. S.} and Nitza Cintron and Chen, {Y. M.}",
year = "1989",
month = "11",
doi = "10.1007/BF02261068",
language = "English (US)",
volume = "28",
pages = "497--501",
journal = "Chromatographia",
issn = "0009-5893",
publisher = "Springer Vieweg",
number = "9-10",

}

TY - JOUR

T1 - Isolation and quantitative analysis of hydroxylysine glycosides

AU - Napoli, R. M.

AU - Middleditch, B. S.

AU - Cintron, Nitza

AU - Chen, Y. M.

PY - 1989/11

Y1 - 1989/11

N2 - Methods are described for the isolation of galactosyl hydroxylysine and glucosylgalactosyl hydroxylysine from collagen and for the quantitative analysis of these hydroxylysine glycosides. The isolation procedure, based upon gel filtration and preparative ionpaired reversed-phase high-performance liquid chromatography, is simple and rapid in comparison with existing methods, allowing the production of tens of milligrams of each glycoside in a single day. Quantitative analysis of the hydroxylysine glycosides is effected by reversed-phase high-performance liquid chromatography with precolumn derivatization (using o-phthal-dialdehyde) and fluorescence detection.

AB - Methods are described for the isolation of galactosyl hydroxylysine and glucosylgalactosyl hydroxylysine from collagen and for the quantitative analysis of these hydroxylysine glycosides. The isolation procedure, based upon gel filtration and preparative ionpaired reversed-phase high-performance liquid chromatography, is simple and rapid in comparison with existing methods, allowing the production of tens of milligrams of each glycoside in a single day. Quantitative analysis of the hydroxylysine glycosides is effected by reversed-phase high-performance liquid chromatography with precolumn derivatization (using o-phthal-dialdehyde) and fluorescence detection.

KW - Amino acid derivatives

KW - Collagen metabolites

KW - Column liquid chromatography

KW - Glycosides

UR - http://www.scopus.com/inward/record.url?scp=0344853832&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0344853832&partnerID=8YFLogxK

U2 - 10.1007/BF02261068

DO - 10.1007/BF02261068

M3 - Article

AN - SCOPUS:0344853832

VL - 28

SP - 497

EP - 501

JO - Chromatographia

JF - Chromatographia

SN - 0009-5893

IS - 9-10

ER -