Isolation of a strain of a Hantaan virus from a fatal case of hemorrhagic fever with renal syndrome in Slovenia

T. Avsic-Zupanc, M. Poljak, P. Furlan, R. Kaps, S. Y. Xiao, James LeDuc

Research output: Contribution to journalArticle

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Abstract

Hemorrhagic fever with renal syndrome (HFRS) has been serologically confirmed in Slovenia during the last seven years. There is evidence that three hantaviruses (Hantaan, Puumala, and a newly described form termed Dobrava) circulate simultaneously in this area. Recently, a hantavirus was isolated from the urine and brain tissue of a fatal case of HFRS. Positive immunofluorescent reactions with reference human sera and monoclonal antibodies were first recognized after the second cell culture passage. Extensive cross-reactivity between our isolate and prototype Hantaan virus, strain 76-118, and Hantaan-like isolates from the former Yugoslavia, Fojnica and Plitvice, was revealed by enzyme-linked immunosorbent assay with specific rat antisera. The reaction pattern of the isolate was similar to the prototype Hantaan virus by indirect immunofluorescent assay with a panel of monoclonal antibodies. Furthermore, the specificity of the isolates was confirmed by analysis of polymerase chain reaction products of this virus with five restriction endonucleases. This appears to be the first isolation of a strain of prototype Hantaan virus from a fatal case of HFRS in Europe.

Original languageEnglish (US)
Pages (from-to)393-400
Number of pages8
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume51
Issue number4
StatePublished - 1994
Externally publishedYes

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Hantaan virus
Hemorrhagic Fever with Renal Syndrome
Slovenia
Hantavirus
Monoclonal Antibodies
Yugoslavia
DNA Restriction Enzymes
Immune Sera
Cell Culture Techniques
Enzyme-Linked Immunosorbent Assay
Urine
Viruses
Polymerase Chain Reaction
Brain
Serum

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

Isolation of a strain of a Hantaan virus from a fatal case of hemorrhagic fever with renal syndrome in Slovenia. / Avsic-Zupanc, T.; Poljak, M.; Furlan, P.; Kaps, R.; Xiao, S. Y.; LeDuc, James.

In: American Journal of Tropical Medicine and Hygiene, Vol. 51, No. 4, 1994, p. 393-400.

Research output: Contribution to journalArticle

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AU - Xiao, S. Y.

AU - LeDuc, James

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