Isolation of an egg-laying hormone-binding protein from the gonad of Aplysia californica and its localization in oocytes

J. V A Choate, T. E. Kruger, Maria Micci, J. E. Blankenship

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

A protein solubilized from a membrane preparation of the gonad of Aplysia californica has been isolated by affinity chromatography, using bag cell egg-laying hormone (ELH) as the bound ligand, and partially purified and characterized by gel electrophoresis. The protein has an apparent molecular weight of 52 kDa and consists of two disulfide-linked subunits of about 30 kDa each. The protein is glycosylated and has an acidic pI. Approximately 10-15 μg of this protein can be isolated from a single ovotestis, representing less than 1% of the total protein in the gonad; but the protein could not be detected in buccal mass or body wall, tissues which do not have apparent response to ELH. Antibodies generated against this ELH-binding protein (ELHBP) were used to localize sites in the ovotestis which might contain this molecule and thus represent targets for egg-laying hormone. Immunocytochemical results indicate that the oocytes are a rich source of this protein, since their cytoplasm was the only detectable site of immunoreactivity. Whether this binding protein represents an egg-laying hormone receptor is uncertain, but its prevalence in oocytes suggests that ELH plays a signaling role on these gametes.

Original languageEnglish (US)
Pages (from-to)475-483
Number of pages9
JournalJournal of Comparative Physiology A
Volume173
Issue number4
DOIs
StatePublished - Oct 1993

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Keywords

  • Aplysia
  • Binding protein
  • Egg laying hormone
  • Immunocytochemistry
  • Reproductive system

ASJC Scopus subject areas

  • Behavioral Neuroscience
  • Neuroscience(all)
  • Physiology (medical)
  • Physiology
  • Animal Science and Zoology

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