Isolation of cationic peptides from rat polymorphonuclear leukocyte granule contents using fast protein liquid chromatography

Michael J. Loeffelholz, Malcolm C. Modrzakowski

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Separation of extracted rat polymorphonuclear leukocyte (PMN) granule contents using fast protein liquid chromatography yielded four major protein fractions. These fractions consisted of myeloperoxidase (peak A), neutral protease (peak B), lysozyme (peak C), and low molecular weight, cationic peptides (peak D). This study represents the first noted purification of the cationic peptides of rat PMN granules.

Original languageEnglish (US)
Pages (from-to)377-381
Number of pages5
JournalAnalytical Biochemistry
Volume158
Issue number2
DOIs
StatePublished - Nov 1 1986
Externally publishedYes

Fingerprint

Liquid chromatography
Liquid Chromatography
Rats
Neutrophils
Peptides
Muramidase
Peroxidase
Purification
Proteins
Peptide Hydrolases
Molecular Weight
Molecular weight

Keywords

  • antimicrobial factors
  • cationic proteins
  • fast protein liquid chromatography
  • leukocyte granules
  • polymorphonuclear leukocyte
  • rat

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Isolation of cationic peptides from rat polymorphonuclear leukocyte granule contents using fast protein liquid chromatography. / Loeffelholz, Michael J.; Modrzakowski, Malcolm C.

In: Analytical Biochemistry, Vol. 158, No. 2, 01.11.1986, p. 377-381.

Research output: Contribution to journalArticle

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