Isolation of cationic peptides from rat polymorphonuclear leukocyte granule contents using fast protein liquid chromatography

Michael J. Loeffelholz, Malcolm C. Modrzakowski

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Separation of extracted rat polymorphonuclear leukocyte (PMN) granule contents using fast protein liquid chromatography yielded four major protein fractions. These fractions consisted of myeloperoxidase (peak A), neutral protease (peak B), lysozyme (peak C), and low molecular weight, cationic peptides (peak D). This study represents the first noted purification of the cationic peptides of rat PMN granules.

Original languageEnglish (US)
Pages (from-to)377-381
Number of pages5
JournalAnalytical Biochemistry
Volume158
Issue number2
DOIs
StatePublished - Nov 1 1986

    Fingerprint

Keywords

  • antimicrobial factors
  • cationic proteins
  • fast protein liquid chromatography
  • leukocyte granules
  • polymorphonuclear leukocyte
  • rat

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this