Isolation of cationic peptides from rat polymorphonuclear leukocyte granule contents using fast protein liquid chromatography

Michael J. Loeffelholz; Malcolm C. Modrzakowski

doi:10.1016/0003-2697(86)90564-6

Isolation of cationic peptides from rat polymorphonuclear leukocyte granule contents using fast protein liquid chromatography

Michael J. Loeffelholz
, Malcolm C. Modrzakowski

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Separation of extracted rat polymorphonuclear leukocyte (PMN) granule contents using fast protein liquid chromatography yielded four major protein fractions. These fractions consisted of myeloperoxidase (peak A), neutral protease (peak B), lysozyme (peak C), and low molecular weight, cationic peptides (peak D). This study represents the first noted purification of the cationic peptides of rat PMN granules.

Original language	English (US)
Pages (from-to)	377-381
Number of pages	5
Journal	Analytical Biochemistry
Volume	158
Issue number	2
DOIs	https://doi.org/10.1016/0003-2697(86)90564-6
State	Published - Nov 1 1986
Externally published	Yes

Keywords

antimicrobial factors
cationic proteins
fast protein liquid chromatography
leukocyte granules
polymorphonuclear leukocyte
rat

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0003-2697(86)90564-6

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title = "Isolation of cationic peptides from rat polymorphonuclear leukocyte granule contents using fast protein liquid chromatography",

abstract = "Separation of extracted rat polymorphonuclear leukocyte (PMN) granule contents using fast protein liquid chromatography yielded four major protein fractions. These fractions consisted of myeloperoxidase (peak A), neutral protease (peak B), lysozyme (peak C), and low molecular weight, cationic peptides (peak D). This study represents the first noted purification of the cationic peptides of rat PMN granules.",

keywords = "antimicrobial factors, cationic proteins, fast protein liquid chromatography, leukocyte granules, polymorphonuclear leukocyte, rat",

author = "Loeffelholz, \{Michael J.\} and Modrzakowski, \{Malcolm C.\}",

note = "Funding Information: The authors thank the laboratory of Dr. Peter Johnson, Department of Chemistry, Ohio University, for assistance with, and the use of the Pharmacia FPLC system. This work was supported by Ohio University Baker Fund Award 84-12, and by research funds fim the Ohio University College of Osteopathic Medicine.",

year = "1986",

month = nov,

day = "1",

doi = "10.1016/0003-2697(86)90564-6",

language = "English (US)",

volume = "158",

pages = "377--381",

journal = "Analytical Biochemistry",

issn = "0003-2697",

publisher = "Academic Press Inc.",

number = "2",

}

TY - JOUR

T1 - Isolation of cationic peptides from rat polymorphonuclear leukocyte granule contents using fast protein liquid chromatography

AU - Loeffelholz, Michael J.

AU - Modrzakowski, Malcolm C.

N1 - Funding Information: The authors thank the laboratory of Dr. Peter Johnson, Department of Chemistry, Ohio University, for assistance with, and the use of the Pharmacia FPLC system. This work was supported by Ohio University Baker Fund Award 84-12, and by research funds fim the Ohio University College of Osteopathic Medicine.

PY - 1986/11/1

Y1 - 1986/11/1

N2 - Separation of extracted rat polymorphonuclear leukocyte (PMN) granule contents using fast protein liquid chromatography yielded four major protein fractions. These fractions consisted of myeloperoxidase (peak A), neutral protease (peak B), lysozyme (peak C), and low molecular weight, cationic peptides (peak D). This study represents the first noted purification of the cationic peptides of rat PMN granules.

AB - Separation of extracted rat polymorphonuclear leukocyte (PMN) granule contents using fast protein liquid chromatography yielded four major protein fractions. These fractions consisted of myeloperoxidase (peak A), neutral protease (peak B), lysozyme (peak C), and low molecular weight, cationic peptides (peak D). This study represents the first noted purification of the cationic peptides of rat PMN granules.

KW - antimicrobial factors

KW - cationic proteins

KW - fast protein liquid chromatography

KW - leukocyte granules

KW - polymorphonuclear leukocyte

KW - rat

UR - https://www.scopus.com/pages/publications/0023034647

UR - https://www.scopus.com/pages/publications/0023034647#tab=citedBy

U2 - 10.1016/0003-2697(86)90564-6

DO - 10.1016/0003-2697(86)90564-6

M3 - Article

C2 - 3028207

AN - SCOPUS:0023034647

SN - 0003-2697

VL - 158

SP - 377

EP - 381

JO - Analytical Biochemistry

JF - Analytical Biochemistry

IS - 2

ER -

Isolation of cationic peptides from rat polymorphonuclear leukocyte granule contents using fast protein liquid chromatography

Abstract

Keywords

ASJC Scopus subject areas

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