Isolation of flagellated bacteria implicated in Crohn's disease

L. Wayne Duck, Mark R. Walter, Jan Novak, Denise Kelly, Maurizio Tomasi, Yingzi Cong, Charles O. Elson

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

Background: Serologic expression cloning has identified flagellins of the intestinal microbiota as immunodominant antigens in experimental colitis in mice and in individuals with Crohn's disease (CD). The present study was done to identify the microbial source of such flagellins. Methods: Using a variety of isolation and culture approaches, a number of previously unknown flagellated bacteria were isolated. Based on 16S ribosomal DNA sequences, these bacteria fall into the family Lachnospiraceae of the phylum Firmicutes. Results: Serum IgG from patients with CD and from mice with colitis reacted to the flagellins of these bacteria, and only their flagellins, whereas serum IgG from controls did not. The sequence of these flagellins demonstrate conserved amino- and carboxy-terminal domains that cluster phylogenetically and have a predicted 3D structure similar to Salmonella fliC, including an intact TLR5 binding site. The flagellin of 1 of these bacteria was likely O-glycosylated. Conclusions: The conserved immune response in both mouse and human to these previously unknown flagellins of the microbiota indicate that they play an important role in host-microbe interactions in the intestine.

Original languageEnglish (US)
Pages (from-to)1191-1201
Number of pages11
JournalInflammatory Bowel Diseases
Volume13
Issue number10
DOIs
StatePublished - Oct 2007
Externally publishedYes

Fingerprint

Flagellin
Crohn Disease
Bacteria
Colitis
Immunoglobulin G
Immunodominant Epitopes
Microbiota
Ribosomal DNA
Serum
Salmonella
Intestines
Organism Cloning
Binding Sites

Keywords

  • Antibody
  • Bacteria
  • Flagellin
  • Microbiota
  • Phylogeny

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Duck, L. W., Walter, M. R., Novak, J., Kelly, D., Tomasi, M., Cong, Y., & Elson, C. O. (2007). Isolation of flagellated bacteria implicated in Crohn's disease. Inflammatory Bowel Diseases, 13(10), 1191-1201. https://doi.org/10.1002/ibd.20237

Isolation of flagellated bacteria implicated in Crohn's disease. / Duck, L. Wayne; Walter, Mark R.; Novak, Jan; Kelly, Denise; Tomasi, Maurizio; Cong, Yingzi; Elson, Charles O.

In: Inflammatory Bowel Diseases, Vol. 13, No. 10, 10.2007, p. 1191-1201.

Research output: Contribution to journalArticle

Duck, LW, Walter, MR, Novak, J, Kelly, D, Tomasi, M, Cong, Y & Elson, CO 2007, 'Isolation of flagellated bacteria implicated in Crohn's disease', Inflammatory Bowel Diseases, vol. 13, no. 10, pp. 1191-1201. https://doi.org/10.1002/ibd.20237
Duck, L. Wayne ; Walter, Mark R. ; Novak, Jan ; Kelly, Denise ; Tomasi, Maurizio ; Cong, Yingzi ; Elson, Charles O. / Isolation of flagellated bacteria implicated in Crohn's disease. In: Inflammatory Bowel Diseases. 2007 ; Vol. 13, No. 10. pp. 1191-1201.
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