Isolation of fungal rDNA from bottlenose dolphin skin infected with Loboo loboi

E. M. Haubold, Judith Aronson, D. F. Cowan, M. R. McGinnis

Research output: Contribution to journalArticle

Abstract

Fungal-specific primers targeted for highly conserved genomic nucleic acid sequences were used in a polymerase chain reaction (PCR) to amplify DNA from lobomycosis lesions in a bottlenose dolphin. Sequence alignments of this DNA possessed high homology to fungal ribosomal DNA sequences found in the genus Cladosporium. When used for in situ hybridization, the riboprobe transcribed from a cloned PCRgenerated fragment bound to Loboa loboi cells. These results support the hypothesis that L. loboi in dolphin tissue is a fungus.

Original languageEnglish (US)
Pages (from-to)263-267
Number of pages5
JournalJournal of Medical and Veterinary Mycology
Volume36
Issue number5
StatePublished - 1998

Fingerprint

Bottle-Nosed Dolphin
Ribosomal DNA
Lobomycosis
Fungal DNA
Dolphins
Cladosporium
Skin
Sequence Alignment
DNA
Nucleic Acids
In Situ Hybridization
Fungi
Polymerase Chain Reaction

Keywords

  • Atlantic bottlenose dolphin
  • Fungus
  • Lobo's disease
  • Loboa loboi

ASJC Scopus subject areas

  • Microbiology

Cite this

Isolation of fungal rDNA from bottlenose dolphin skin infected with Loboo loboi. / Haubold, E. M.; Aronson, Judith; Cowan, D. F.; McGinnis, M. R.

In: Journal of Medical and Veterinary Mycology, Vol. 36, No. 5, 1998, p. 263-267.

Research output: Contribution to journalArticle

Haubold, E. M. ; Aronson, Judith ; Cowan, D. F. ; McGinnis, M. R. / Isolation of fungal rDNA from bottlenose dolphin skin infected with Loboo loboi. In: Journal of Medical and Veterinary Mycology. 1998 ; Vol. 36, No. 5. pp. 263-267.
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