Isolation of fungal rDNA from bottlenose dolphin skin infected with Loboa loboi

E. M. Haubold, Judith Aronson, D. F. Cowan, M. R. McGinnis, C. R. Cooper

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Fungal-specific primers targeted for highly conserved genomic nucleic acid sequences were used in a polymerase chain reaction (PCR) to amplify DNA from lobomycosis lesions in a bottlenose dolphin. Sequence alignments of this DNA possessed high homology to fungal ribosomal DNA sequences found in the genus Cladosporium. When used for in situ hybridization, the riboprobe transcribed from a cloned PCR-generated fragment bound to Loboa loboi cells. These results support the hypothesis that L. loboi in dolphin tissue is a fungus.

Original languageEnglish (US)
Pages (from-to)263-267
Number of pages5
JournalMedical Mycology
Volume36
Issue number5
StatePublished - Oct 1998

Fingerprint

Bottle-Nosed Dolphin
Tursiops truncatus
Ribosomal DNA
Lobomycosis
Fungal DNA
polymerase chain reaction
Dolphins
Cladosporium
Polymerase Chain Reaction
Skin
Sequence Alignment
DNA
sequence alignment
dolphins
ribosomal DNA
in situ hybridization
Nucleic Acids
nucleic acids
In Situ Hybridization
Fungi

Keywords

  • Atlantic bottlenose dolphin
  • Fungus
  • Lobo's disease
  • Loboa loboi

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • veterinary(all)
  • Microbiology

Cite this

Haubold, E. M., Aronson, J., Cowan, D. F., McGinnis, M. R., & Cooper, C. R. (1998). Isolation of fungal rDNA from bottlenose dolphin skin infected with Loboa loboi. Medical Mycology, 36(5), 263-267.

Isolation of fungal rDNA from bottlenose dolphin skin infected with Loboa loboi. / Haubold, E. M.; Aronson, Judith; Cowan, D. F.; McGinnis, M. R.; Cooper, C. R.

In: Medical Mycology, Vol. 36, No. 5, 10.1998, p. 263-267.

Research output: Contribution to journalArticle

Haubold, EM, Aronson, J, Cowan, DF, McGinnis, MR & Cooper, CR 1998, 'Isolation of fungal rDNA from bottlenose dolphin skin infected with Loboa loboi', Medical Mycology, vol. 36, no. 5, pp. 263-267.
Haubold EM, Aronson J, Cowan DF, McGinnis MR, Cooper CR. Isolation of fungal rDNA from bottlenose dolphin skin infected with Loboa loboi. Medical Mycology. 1998 Oct;36(5):263-267.
Haubold, E. M. ; Aronson, Judith ; Cowan, D. F. ; McGinnis, M. R. ; Cooper, C. R. / Isolation of fungal rDNA from bottlenose dolphin skin infected with Loboa loboi. In: Medical Mycology. 1998 ; Vol. 36, No. 5. pp. 263-267.
@article{672d7eb6fb9c4a14b28f5bf10c87c8b1,
title = "Isolation of fungal rDNA from bottlenose dolphin skin infected with Loboa loboi",
abstract = "Fungal-specific primers targeted for highly conserved genomic nucleic acid sequences were used in a polymerase chain reaction (PCR) to amplify DNA from lobomycosis lesions in a bottlenose dolphin. Sequence alignments of this DNA possessed high homology to fungal ribosomal DNA sequences found in the genus Cladosporium. When used for in situ hybridization, the riboprobe transcribed from a cloned PCR-generated fragment bound to Loboa loboi cells. These results support the hypothesis that L. loboi in dolphin tissue is a fungus.",
keywords = "Atlantic bottlenose dolphin, Fungus, Lobo's disease, Loboa loboi",
author = "Haubold, {E. M.} and Judith Aronson and Cowan, {D. F.} and McGinnis, {M. R.} and Cooper, {C. R.}",
year = "1998",
month = "10",
language = "English (US)",
volume = "36",
pages = "263--267",
journal = "Medical Mycology",
issn = "1369-3786",
publisher = "Informa Healthcare",
number = "5",

}

TY - JOUR

T1 - Isolation of fungal rDNA from bottlenose dolphin skin infected with Loboa loboi

AU - Haubold, E. M.

AU - Aronson, Judith

AU - Cowan, D. F.

AU - McGinnis, M. R.

AU - Cooper, C. R.

PY - 1998/10

Y1 - 1998/10

N2 - Fungal-specific primers targeted for highly conserved genomic nucleic acid sequences were used in a polymerase chain reaction (PCR) to amplify DNA from lobomycosis lesions in a bottlenose dolphin. Sequence alignments of this DNA possessed high homology to fungal ribosomal DNA sequences found in the genus Cladosporium. When used for in situ hybridization, the riboprobe transcribed from a cloned PCR-generated fragment bound to Loboa loboi cells. These results support the hypothesis that L. loboi in dolphin tissue is a fungus.

AB - Fungal-specific primers targeted for highly conserved genomic nucleic acid sequences were used in a polymerase chain reaction (PCR) to amplify DNA from lobomycosis lesions in a bottlenose dolphin. Sequence alignments of this DNA possessed high homology to fungal ribosomal DNA sequences found in the genus Cladosporium. When used for in situ hybridization, the riboprobe transcribed from a cloned PCR-generated fragment bound to Loboa loboi cells. These results support the hypothesis that L. loboi in dolphin tissue is a fungus.

KW - Atlantic bottlenose dolphin

KW - Fungus

KW - Lobo's disease

KW - Loboa loboi

UR - http://www.scopus.com/inward/record.url?scp=0031647857&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031647857&partnerID=8YFLogxK

M3 - Article

C2 - 10075494

AN - SCOPUS:0031647857

VL - 36

SP - 263

EP - 267

JO - Medical Mycology

JF - Medical Mycology

SN - 1369-3786

IS - 5

ER -