Abstract
Fungal-specific primers targeted for highly conserved genomic nucleic acid sequences were used in a polymerase chain reaction (PCR) to amplify DNA from lobomycosis lesions in a bottlenose dolphin. Sequence alignments of this DNA possessed high homology to fungal ribosomal DNA sequences found in the genus Cladosporium. When used for in situ hybridization, the riboprobe transcribed from a cloned PCR-generated fragment bound to Loboa loboi cells. These results support the hypothesis that L. loboi in dolphin tissue is a fungus.
Original language | English (US) |
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Pages (from-to) | 263-267 |
Number of pages | 5 |
Journal | Medical Mycology |
Volume | 36 |
Issue number | 5 |
DOIs | |
State | Published - Oct 1998 |
Keywords
- Atlantic bottlenose dolphin
- Fungus
- Lobo's disease
- Loboa loboi
ASJC Scopus subject areas
- Infectious Diseases