Isolation of fungal rDNA from bottlenose dolphin skin infected with Loboa loboi

E. M. Haubold, J. F. Aronson, D. F. Cowan, M. R. McGinnis, C. R. Cooper

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

Fungal-specific primers targeted for highly conserved genomic nucleic acid sequences were used in a polymerase chain reaction (PCR) to amplify DNA from lobomycosis lesions in a bottlenose dolphin. Sequence alignments of this DNA possessed high homology to fungal ribosomal DNA sequences found in the genus Cladosporium. When used for in situ hybridization, the riboprobe transcribed from a cloned PCR-generated fragment bound to Loboa loboi cells. These results support the hypothesis that L. loboi in dolphin tissue is a fungus.

Original languageEnglish (US)
Pages (from-to)263-267
Number of pages5
JournalMedical Mycology
Volume36
Issue number5
DOIs
StatePublished - Oct 1998

Keywords

  • Atlantic bottlenose dolphin
  • Fungus
  • Lobo's disease
  • Loboa loboi

ASJC Scopus subject areas

  • Infectious Diseases

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