Isolation of Kaeng Khoi virus from dead Chaerephon plicata bats in Cambodia

J. C. Osborne, C. E. Rupprecht, J. G. Olson, Thomas Ksiazek, P. E. Rollin, M. Niezgoda, C. S. Goldsmith, U. S. An, Stuart T. Nichol

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

A virus isolated from dead Chaerephon plicata bats collected near Kampot, Cambodia, was identified as a member of the family Bunyaviridae by electron microscopy. The only bunyavirus previously isolated from Chaerephon species bats in South-East Asia is Kaeng Khoi (KK) virus (genus Orthobunyavirus), detected in Thailand over 30 years earlier and implicated as a public health problem. Using RT-PCR, nucleotide sequences from the M RNA segment of several virus isolates from the Cambodian C, plicata bats were found to be almost identical and to differ from those of the prototype KK virus by only 2.6-3.2%, despite the temporal and geographic separation of the viruses. These results identify the Cambodian bat viruses as KK virus, extend the known virus geographic range and document the first KK virus isolation in 30 years. These genetic data, together with earlier serologic data, show that KK viruses represent a distinct group within the genus Orthobunyavirus.

Original languageEnglish (US)
Pages (from-to)2685-2689
Number of pages5
JournalJournal of General Virology
Volume84
Issue number10
DOIs
StatePublished - Oct 1 2003
Externally publishedYes

Fingerprint

Cambodia
Viruses
Orthobunyavirus
Bunyaviridae
Far East
Thailand
Electron Microscopy
Public Health
RNA

ASJC Scopus subject areas

  • Virology
  • Immunology

Cite this

Osborne, J. C., Rupprecht, C. E., Olson, J. G., Ksiazek, T., Rollin, P. E., Niezgoda, M., ... Nichol, S. T. (2003). Isolation of Kaeng Khoi virus from dead Chaerephon plicata bats in Cambodia. Journal of General Virology, 84(10), 2685-2689. https://doi.org/10.1099/vir.0.19294-0

Isolation of Kaeng Khoi virus from dead Chaerephon plicata bats in Cambodia. / Osborne, J. C.; Rupprecht, C. E.; Olson, J. G.; Ksiazek, Thomas; Rollin, P. E.; Niezgoda, M.; Goldsmith, C. S.; An, U. S.; Nichol, Stuart T.

In: Journal of General Virology, Vol. 84, No. 10, 01.10.2003, p. 2685-2689.

Research output: Contribution to journalArticle

Osborne, JC, Rupprecht, CE, Olson, JG, Ksiazek, T, Rollin, PE, Niezgoda, M, Goldsmith, CS, An, US & Nichol, ST 2003, 'Isolation of Kaeng Khoi virus from dead Chaerephon plicata bats in Cambodia', Journal of General Virology, vol. 84, no. 10, pp. 2685-2689. https://doi.org/10.1099/vir.0.19294-0
Osborne, J. C. ; Rupprecht, C. E. ; Olson, J. G. ; Ksiazek, Thomas ; Rollin, P. E. ; Niezgoda, M. ; Goldsmith, C. S. ; An, U. S. ; Nichol, Stuart T. / Isolation of Kaeng Khoi virus from dead Chaerephon plicata bats in Cambodia. In: Journal of General Virology. 2003 ; Vol. 84, No. 10. pp. 2685-2689.
@article{53895d9a248a4070bccfc9c81fa050e4,
title = "Isolation of Kaeng Khoi virus from dead Chaerephon plicata bats in Cambodia",
abstract = "A virus isolated from dead Chaerephon plicata bats collected near Kampot, Cambodia, was identified as a member of the family Bunyaviridae by electron microscopy. The only bunyavirus previously isolated from Chaerephon species bats in South-East Asia is Kaeng Khoi (KK) virus (genus Orthobunyavirus), detected in Thailand over 30 years earlier and implicated as a public health problem. Using RT-PCR, nucleotide sequences from the M RNA segment of several virus isolates from the Cambodian C, plicata bats were found to be almost identical and to differ from those of the prototype KK virus by only 2.6-3.2{\%}, despite the temporal and geographic separation of the viruses. These results identify the Cambodian bat viruses as KK virus, extend the known virus geographic range and document the first KK virus isolation in 30 years. These genetic data, together with earlier serologic data, show that KK viruses represent a distinct group within the genus Orthobunyavirus.",
author = "Osborne, {J. C.} and Rupprecht, {C. E.} and Olson, {J. G.} and Thomas Ksiazek and Rollin, {P. E.} and M. Niezgoda and Goldsmith, {C. S.} and An, {U. S.} and Nichol, {Stuart T.}",
year = "2003",
month = "10",
day = "1",
doi = "10.1099/vir.0.19294-0",
language = "English (US)",
volume = "84",
pages = "2685--2689",
journal = "Journal of General Virology",
issn = "0022-1317",
publisher = "Society for General Microbiology",
number = "10",

}

TY - JOUR

T1 - Isolation of Kaeng Khoi virus from dead Chaerephon plicata bats in Cambodia

AU - Osborne, J. C.

AU - Rupprecht, C. E.

AU - Olson, J. G.

AU - Ksiazek, Thomas

AU - Rollin, P. E.

AU - Niezgoda, M.

AU - Goldsmith, C. S.

AU - An, U. S.

AU - Nichol, Stuart T.

PY - 2003/10/1

Y1 - 2003/10/1

N2 - A virus isolated from dead Chaerephon plicata bats collected near Kampot, Cambodia, was identified as a member of the family Bunyaviridae by electron microscopy. The only bunyavirus previously isolated from Chaerephon species bats in South-East Asia is Kaeng Khoi (KK) virus (genus Orthobunyavirus), detected in Thailand over 30 years earlier and implicated as a public health problem. Using RT-PCR, nucleotide sequences from the M RNA segment of several virus isolates from the Cambodian C, plicata bats were found to be almost identical and to differ from those of the prototype KK virus by only 2.6-3.2%, despite the temporal and geographic separation of the viruses. These results identify the Cambodian bat viruses as KK virus, extend the known virus geographic range and document the first KK virus isolation in 30 years. These genetic data, together with earlier serologic data, show that KK viruses represent a distinct group within the genus Orthobunyavirus.

AB - A virus isolated from dead Chaerephon plicata bats collected near Kampot, Cambodia, was identified as a member of the family Bunyaviridae by electron microscopy. The only bunyavirus previously isolated from Chaerephon species bats in South-East Asia is Kaeng Khoi (KK) virus (genus Orthobunyavirus), detected in Thailand over 30 years earlier and implicated as a public health problem. Using RT-PCR, nucleotide sequences from the M RNA segment of several virus isolates from the Cambodian C, plicata bats were found to be almost identical and to differ from those of the prototype KK virus by only 2.6-3.2%, despite the temporal and geographic separation of the viruses. These results identify the Cambodian bat viruses as KK virus, extend the known virus geographic range and document the first KK virus isolation in 30 years. These genetic data, together with earlier serologic data, show that KK viruses represent a distinct group within the genus Orthobunyavirus.

UR - http://www.scopus.com/inward/record.url?scp=0142025054&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0142025054&partnerID=8YFLogxK

U2 - 10.1099/vir.0.19294-0

DO - 10.1099/vir.0.19294-0

M3 - Article

VL - 84

SP - 2685

EP - 2689

JO - Journal of General Virology

JF - Journal of General Virology

SN - 0022-1317

IS - 10

ER -