Isolation of mesenchymal stem cells from umbilical cord (method)

Katsuhiro Kita; Gerd G. Gauglitz; Stephen Williamson; David N. Herndon; Thang T. Phan; Marc G. Jeschke

doi:10.1007/978-94-007-2415-0_3

Isolation of mesenchymal stem cells from umbilical cord (method)

Katsuhiro Kita, Gerd G. Gauglitz, Stephen Williamson, David N. Herndon, Thang T. Phan, Marc G. Jeschke

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

Recent advances in stem cell (SC) research have attracted many biomedical researchers to explore the potential of SCs as new biomaterials. In addition to regenerative activity, SCs are also useful for high throughput screening and safety test of human drug targets. Although embryonic SCs possess best potential in terms of multipotency, their use has remained widely controversial due to ethical concerns. Meanwhile, intensive studies on adult SCs in the last decade have given us an alternative option as represented by mesenchymal SCs (MSCs). We recently reported successful isolation and characterization ofMSCs from human umbilical cord lining membrane (sub-amnion). In this chapter, we would like to provide general guidelines for the isolation of MSCs from umbilical cord.

Original language	English (US)
Title of host publication	Stem Cells and Cancer Stem Cells, Volume 3
Subtitle of host publication	Stem Cells and Cancer Stem Cells, Therapeutic Applications in Disease and Injury: Volume 3
Publisher	Springer Netherlands
Pages	15-21
Number of pages	7
ISBN (Electronic)	9789400724150
ISBN (Print)	9789400724143
DOIs	https://doi.org/10.1007/978-94-007-2415-0_3
State	Published - Jan 1 2012
Externally published	Yes

Keywords

Amnion
Isolation
Mesenchymal SCs
Multipotent cells
Mycoplasma
Umbilical cord

ASJC Scopus subject areas

General Medicine

Access to Document

10.1007/978-94-007-2415-0_3

Cite this

Kita, K., Gauglitz, G. G., Williamson, S., Herndon, D. N., Phan, T. T., & Jeschke, M. G. (2012). Isolation of mesenchymal stem cells from umbilical cord (method). In Stem Cells and Cancer Stem Cells, Volume 3: Stem Cells and Cancer Stem Cells, Therapeutic Applications in Disease and Injury: Volume 3 (pp. 15-21). Springer Netherlands. https://doi.org/10.1007/978-94-007-2415-0_3

Isolation of mesenchymal stem cells from umbilical cord (method). / Kita, Katsuhiro; Gauglitz, Gerd G.; Williamson, Stephen et al.
Stem Cells and Cancer Stem Cells, Volume 3: Stem Cells and Cancer Stem Cells, Therapeutic Applications in Disease and Injury: Volume 3. Springer Netherlands, 2012. p. 15-21.

Research output: Chapter in Book/Report/Conference proceeding › Chapter

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Isolation of mesenchymal stem cells from umbilical cord (method)

Abstract

Keywords

ASJC Scopus subject areas

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