Isoproterenol inhibits IL-10, TNF-α, and nitric oxide production in RAW 264.7 Macrophages

György Haskó, Zoltán H. Németh, Csaba Szabó, Gabriella Zsilla, Andrew L. Salzman, E. Sylvester Vizi

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    Abstract

    In a previous study we have demonstrated in conscious endotoxemic mice that isoproterenol, a nonselective agonist of β-adrenergic receptors, decreased the production of proinflammatory mediators tumor necrosis factor- α (TNF-α) and nitric oxide (NO), and enhanced the formation of the anti- inflammatory cytokine interleukin-10 (IL-10). In the present study we investigated the effect of isoproterenol on the bacterial lipopolysaccharide (endotoxin, LPS; 10 μg/ml)-induced inflammatory response in RAW 264.7 macrophages in vitro. Pretreatment of cells with isoproterenol (10-300 μM) resulted in an inhibition of TNF-α, NO (reflected as its stable breakdown product nitrite), as well as IL-10 production that was paralleled with a restoration of the LPS-induced suppression of mitochondrial respiration. In addition, isoproterenol elevated cAMP accumulation in these cells. Finally, isoproterenol (300 μM) did not influence the nuclear translocation of nuclear factor κB. These data demonstrate that isoproterenol potently downregulates the LPS-induced inflammatory response and further support the notion that stimulation of β-adrenoreceptors can be an effective strategy in the treatment of inflammatory diseases.

    Original languageEnglish (US)
    Pages (from-to)183-187
    Number of pages5
    JournalBrain Research Bulletin
    Volume45
    Issue number2
    DOIs
    StatePublished - Jan 20 1998

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    Keywords

    • Catecholamines
    • Cyclic adenosine monophosphate
    • Cytokines
    • Inflammation
    • Lipopolysaccharide
    • Nitric Oxide
    • Septic shock
    • β-Adrenoceptors

    ASJC Scopus subject areas

    • Neuroscience(all)

    Cite this

    Haskó, G., Németh, Z. H., Szabó, C., Zsilla, G., Salzman, A. L., & Vizi, E. S. (1998). Isoproterenol inhibits IL-10, TNF-α, and nitric oxide production in RAW 264.7 Macrophages. Brain Research Bulletin, 45(2), 183-187. https://doi.org/10.1016/S0361-9230(97)00337-7