Isotopic decay of urinary or plasma 3-methylhistidine as a potential biomarker of pathologic skeletal muscle loss

M. Sheffield-Moore, Edgar Dillon, K. M. Randolph, S. L. Casperson, G. R. White, Kristofer Jennings, J. Rathmacher, S. Schuette, M. Janghorbani, Randall Urban, V. Hoang, Maurice Willis, W. J. Durham

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Background: Skeletal muscle loss accompanying aging or cancer is associated with reduced physical function and predicts morbidity and mortality. 3-Methylhistidine (3MH) has been proposed as a biomarker of myofibrillar proteolysis, which may contribute to skeletal muscle loss. Methods: We hypothesized that the terminal portion of the isotope decay curve following an oral dose of isotopically labeled 3MH can be measured non-invasively from timed spot urine samples. We investigated the feasibility of this approach by determining isotope enrichment in spot urine samples and corresponding plasma samples and whether meat intake up to the time of dosing influences the isotope decay. Results: Isotope decay constants (k) were similar in plasma and urine, regardless of diet. Post hoc comparison of hourly sampling over 10 h with three samples distributed over 10 or fewer hours suggests that three distributed samples over 5-6 h of plasma or urine sampling yield decay constants similar to those obtained over 10 h of hourly sampling. Conclusion: The findings from this study suggest that an index of 3MH production can be obtained from an easily administered test involving oral administration of a stable isotope tracer of 3MH followed by three plasma or urine samples collected over 5-6 h the next day.

Original languageEnglish (US)
Pages (from-to)19-25
Number of pages7
JournalJournal of Cachexia, Sarcopenia and Muscle
Volume5
Issue number1
DOIs
StatePublished - 2014

Fingerprint

Isotopes
Skeletal Muscle
Biomarkers
Urine
Meat
Proteolysis
Oral Administration
3-methylhistidine
Diet
Morbidity
Mortality
Neoplasms

Keywords

  • Cachexia
  • Human
  • Sarcopenia
  • Tracer

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine
  • Physiology (medical)

Cite this

Isotopic decay of urinary or plasma 3-methylhistidine as a potential biomarker of pathologic skeletal muscle loss. / Sheffield-Moore, M.; Dillon, Edgar; Randolph, K. M.; Casperson, S. L.; White, G. R.; Jennings, Kristofer; Rathmacher, J.; Schuette, S.; Janghorbani, M.; Urban, Randall; Hoang, V.; Willis, Maurice; Durham, W. J.

In: Journal of Cachexia, Sarcopenia and Muscle, Vol. 5, No. 1, 2014, p. 19-25.

Research output: Contribution to journalArticle

Sheffield-Moore, M, Dillon, E, Randolph, KM, Casperson, SL, White, GR, Jennings, K, Rathmacher, J, Schuette, S, Janghorbani, M, Urban, R, Hoang, V, Willis, M & Durham, WJ 2014, 'Isotopic decay of urinary or plasma 3-methylhistidine as a potential biomarker of pathologic skeletal muscle loss', Journal of Cachexia, Sarcopenia and Muscle, vol. 5, no. 1, pp. 19-25. https://doi.org/10.1007/s13539-013-0117-7
Sheffield-Moore, M. ; Dillon, Edgar ; Randolph, K. M. ; Casperson, S. L. ; White, G. R. ; Jennings, Kristofer ; Rathmacher, J. ; Schuette, S. ; Janghorbani, M. ; Urban, Randall ; Hoang, V. ; Willis, Maurice ; Durham, W. J. / Isotopic decay of urinary or plasma 3-methylhistidine as a potential biomarker of pathologic skeletal muscle loss. In: Journal of Cachexia, Sarcopenia and Muscle. 2014 ; Vol. 5, No. 1. pp. 19-25.
@article{38e83f9fbb1c4a65bb018c25c311094e,
title = "Isotopic decay of urinary or plasma 3-methylhistidine as a potential biomarker of pathologic skeletal muscle loss",
abstract = "Background: Skeletal muscle loss accompanying aging or cancer is associated with reduced physical function and predicts morbidity and mortality. 3-Methylhistidine (3MH) has been proposed as a biomarker of myofibrillar proteolysis, which may contribute to skeletal muscle loss. Methods: We hypothesized that the terminal portion of the isotope decay curve following an oral dose of isotopically labeled 3MH can be measured non-invasively from timed spot urine samples. We investigated the feasibility of this approach by determining isotope enrichment in spot urine samples and corresponding plasma samples and whether meat intake up to the time of dosing influences the isotope decay. Results: Isotope decay constants (k) were similar in plasma and urine, regardless of diet. Post hoc comparison of hourly sampling over 10 h with three samples distributed over 10 or fewer hours suggests that three distributed samples over 5-6 h of plasma or urine sampling yield decay constants similar to those obtained over 10 h of hourly sampling. Conclusion: The findings from this study suggest that an index of 3MH production can be obtained from an easily administered test involving oral administration of a stable isotope tracer of 3MH followed by three plasma or urine samples collected over 5-6 h the next day.",
keywords = "Cachexia, Human, Sarcopenia, Tracer",
author = "M. Sheffield-Moore and Edgar Dillon and Randolph, {K. M.} and Casperson, {S. L.} and White, {G. R.} and Kristofer Jennings and J. Rathmacher and S. Schuette and M. Janghorbani and Randall Urban and V. Hoang and Maurice Willis and Durham, {W. J.}",
year = "2014",
doi = "10.1007/s13539-013-0117-7",
language = "English (US)",
volume = "5",
pages = "19--25",
journal = "AMB Express",
issn = "2191-0855",
publisher = "Springer Verlag",
number = "1",

}

TY - JOUR

T1 - Isotopic decay of urinary or plasma 3-methylhistidine as a potential biomarker of pathologic skeletal muscle loss

AU - Sheffield-Moore, M.

AU - Dillon, Edgar

AU - Randolph, K. M.

AU - Casperson, S. L.

AU - White, G. R.

AU - Jennings, Kristofer

AU - Rathmacher, J.

AU - Schuette, S.

AU - Janghorbani, M.

AU - Urban, Randall

AU - Hoang, V.

AU - Willis, Maurice

AU - Durham, W. J.

PY - 2014

Y1 - 2014

N2 - Background: Skeletal muscle loss accompanying aging or cancer is associated with reduced physical function and predicts morbidity and mortality. 3-Methylhistidine (3MH) has been proposed as a biomarker of myofibrillar proteolysis, which may contribute to skeletal muscle loss. Methods: We hypothesized that the terminal portion of the isotope decay curve following an oral dose of isotopically labeled 3MH can be measured non-invasively from timed spot urine samples. We investigated the feasibility of this approach by determining isotope enrichment in spot urine samples and corresponding plasma samples and whether meat intake up to the time of dosing influences the isotope decay. Results: Isotope decay constants (k) were similar in plasma and urine, regardless of diet. Post hoc comparison of hourly sampling over 10 h with three samples distributed over 10 or fewer hours suggests that three distributed samples over 5-6 h of plasma or urine sampling yield decay constants similar to those obtained over 10 h of hourly sampling. Conclusion: The findings from this study suggest that an index of 3MH production can be obtained from an easily administered test involving oral administration of a stable isotope tracer of 3MH followed by three plasma or urine samples collected over 5-6 h the next day.

AB - Background: Skeletal muscle loss accompanying aging or cancer is associated with reduced physical function and predicts morbidity and mortality. 3-Methylhistidine (3MH) has been proposed as a biomarker of myofibrillar proteolysis, which may contribute to skeletal muscle loss. Methods: We hypothesized that the terminal portion of the isotope decay curve following an oral dose of isotopically labeled 3MH can be measured non-invasively from timed spot urine samples. We investigated the feasibility of this approach by determining isotope enrichment in spot urine samples and corresponding plasma samples and whether meat intake up to the time of dosing influences the isotope decay. Results: Isotope decay constants (k) were similar in plasma and urine, regardless of diet. Post hoc comparison of hourly sampling over 10 h with three samples distributed over 10 or fewer hours suggests that three distributed samples over 5-6 h of plasma or urine sampling yield decay constants similar to those obtained over 10 h of hourly sampling. Conclusion: The findings from this study suggest that an index of 3MH production can be obtained from an easily administered test involving oral administration of a stable isotope tracer of 3MH followed by three plasma or urine samples collected over 5-6 h the next day.

KW - Cachexia

KW - Human

KW - Sarcopenia

KW - Tracer

UR - http://www.scopus.com/inward/record.url?scp=84896357087&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84896357087&partnerID=8YFLogxK

U2 - 10.1007/s13539-013-0117-7

DO - 10.1007/s13539-013-0117-7

M3 - Article

VL - 5

SP - 19

EP - 25

JO - AMB Express

JF - AMB Express

SN - 2191-0855

IS - 1

ER -