Ixodes scapularis salivary gland microRNAs are differentially expressed during Powassan virus transmission

Meghan E. Hermance, Steven Widen, Thomas G. Wood, Saravanan Thangamani

Research output: Contribution to journalArticle

Abstract

Successful tick feeding is facilitated by an assortment of pharmacologically-active factors in tick saliva that create an immunologically privileged micro-environment in the host's skin. Through a process known as saliva-assisted transmission, bioactive tick salivary factors modulate the host environment, promoting transmission and establishment of a tick-borne pathogen. This phenomenon was previously demonstrated for Powassan virus (POWV), a North American tick-borne flavivirus that is the causative agent of a severe neuroinvasive disease in humans. Here, we sought to characterize the Ixodes scapularis salivary gland microRNAs (miRNAs) expressed during the earliest period of POWV transmission to a mammalian host. POWV-infected and uninfected I. scapularis females were fed on naïve mice for 1, 3, and 6 hours, and Illumina next generation sequencing was used to characterize the salivary gland miRNA expression profiles of POWV-infected versus uninfected ticks. 379 salivary miRNAs were detected, of which 338 are reported here as putative novel I. scapularis miRNAs. 35 salivary gland miRNAs were significantly up-regulated and 17 miRNAs were significantly down-regulated in response to POWV infection. To investigate the potential role of salivary gland miRNAs in POWV replication in-vitro, we transfected miRNA inhibitors into VeroE6 cells to profile temporal POWV replication in mammalian cells. Together, the small RNA sequencing data and the in vitro miRNA inhibition assay suggest that the differentially expressed tick salivary miRNAs could act in regulating POWV replication in host tissues.

Original languageEnglish (US)
Number of pages1
JournalScientific reports
Volume9
Issue number1
DOIs
StatePublished - Sep 11 2019

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Tick-Borne Encephalitis Viruses
Ixodes
Salivary Glands
MicroRNAs
Ticks
Virus Replication
Saliva
RNA Sequence Analysis
Flavivirus
Virus Diseases

ASJC Scopus subject areas

  • General

Cite this

Ixodes scapularis salivary gland microRNAs are differentially expressed during Powassan virus transmission. / Hermance, Meghan E.; Widen, Steven; Wood, Thomas G.; Thangamani, Saravanan.

In: Scientific reports, Vol. 9, No. 1, 11.09.2019.

Research output: Contribution to journalArticle

Hermance, Meghan E. ; Widen, Steven ; Wood, Thomas G. ; Thangamani, Saravanan. / Ixodes scapularis salivary gland microRNAs are differentially expressed during Powassan virus transmission. In: Scientific reports. 2019 ; Vol. 9, No. 1.
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