Jatobal virus antigenic characterization by ELISA and neutralization test using EIA as indicator, on tissue culture.

L. T. Figueiredo, A. P. Da Rosa

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

A virus antigenic characterization methodology using an indirect method of antibody detection ELISA with virus-infected cultured cells as antigen and a micro virus neutralisation test using EIA (NT-EIA) as an aid to reading were used for antigenic characterization of Jatobal (BeAn 423380). Jatobal virus was characterized as a Bunyaviridae, Bunyavirus genus, Simbu serogroup virus. ELISA using infected cultured cells as antigen is a sensitive and reliable method for identification of viruses and has many advantages over conventional antibody capture ELISA's and other tests: it eliminates solid phase coating with virus and laborious antigen preparation; it permits screening of large numbers of virus antisera faster and more easily than by CF, HAI, or plaque reduction NT. ELISA and NT using EIA as an aid to reading can be applicable to viruses which do not produce cytopathogenic effect. Both techniques are applicable to identification of viruses which grow in mosquito cells.

Original languageEnglish (US)
Pages (from-to)161-164
Number of pages4
JournalMemorias do Instituto Oswaldo Cruz
Volume83
Issue number2
StatePublished - Apr 1988
Externally publishedYes

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Neutralization Tests
Enzyme-Linked Immunosorbent Assay
Viruses
Antigens
Simbu virus
Reading
Cultured Cells
Orthobunyavirus
Bunyaviridae
Antibodies
Culicidae
Immune Sera

ASJC Scopus subject areas

  • Microbiology (medical)

Cite this

Jatobal virus antigenic characterization by ELISA and neutralization test using EIA as indicator, on tissue culture. / Figueiredo, L. T.; Da Rosa, A. P.

In: Memorias do Instituto Oswaldo Cruz, Vol. 83, No. 2, 04.1988, p. 161-164.

Research output: Contribution to journalArticle

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