Kappa opioid receptors of human placental villi modulate acetylcholine release

Mahmoud Ahmed, Timothy Schoof, De He Zhou, Christopher Quarles

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Human placental villus tissue is non-innervated, yet it contains components of the opiate and cholinergic systems. We investigated whether opioids modulate a calcium dependent acetyltholine release from the villus tissue in a manner similar to that demonstrated by the parasympathetic nerve-smooth muscle junction. We reported that the κ receptor agonist ethylketocyclazocine (EKC) inhibits acetylcholine release, and that the inhibition is reversed by the selective antagonist, Mr2266. Findings reported here substantiate the role of opioids as modulators of acetylcholine release from villus tissue. The non-selective agonist, morphine, also inhibits acetylcholine release. Inhibition caused by morphine is reversed by low concentrations of non-selective antagonists, naloxone and naltrexone. Naloxone at high concentrations potentiates the inhibition of acetylcholine release caused by morphine. In addition, the calcium channel blocker, diltiazem, was found to inhibit the release of acetylcholine. The combination of morphine and diltiazem resulted in a greater inhibition of acetylcholine release than by either alone. These results suggest that opiate cholinergic interactions occur in non-neural tissue with a mechanism similar to that known to occur at certain cholinergic synapses.

Original languageEnglish (US)
Pages (from-to)2383-2393
Number of pages11
JournalLife Sciences
Volume45
Issue number25
DOIs
StatePublished - 1989
Externally publishedYes

Fingerprint

Chorionic Villi
kappa Opioid Receptor
Acetylcholine
Morphine
Opiate Alkaloids
Tissue
Cholinergic Agents
Diltiazem
Naloxone
Opioid Analgesics
Ethylketocyclazocine
Naltrexone
Calcium Channel Blockers
Synapses
Modulators
Smooth Muscle
Muscle
Calcium

ASJC Scopus subject areas

  • Pharmacology

Cite this

Kappa opioid receptors of human placental villi modulate acetylcholine release. / Ahmed, Mahmoud; Schoof, Timothy; Zhou, De He; Quarles, Christopher.

In: Life Sciences, Vol. 45, No. 25, 1989, p. 2383-2393.

Research output: Contribution to journalArticle

Ahmed, Mahmoud ; Schoof, Timothy ; Zhou, De He ; Quarles, Christopher. / Kappa opioid receptors of human placental villi modulate acetylcholine release. In: Life Sciences. 1989 ; Vol. 45, No. 25. pp. 2383-2393.
@article{a9366f1cd2474d09b5cb1b2f758382e9,
title = "Kappa opioid receptors of human placental villi modulate acetylcholine release",
abstract = "Human placental villus tissue is non-innervated, yet it contains components of the opiate and cholinergic systems. We investigated whether opioids modulate a calcium dependent acetyltholine release from the villus tissue in a manner similar to that demonstrated by the parasympathetic nerve-smooth muscle junction. We reported that the κ receptor agonist ethylketocyclazocine (EKC) inhibits acetylcholine release, and that the inhibition is reversed by the selective antagonist, Mr2266. Findings reported here substantiate the role of opioids as modulators of acetylcholine release from villus tissue. The non-selective agonist, morphine, also inhibits acetylcholine release. Inhibition caused by morphine is reversed by low concentrations of non-selective antagonists, naloxone and naltrexone. Naloxone at high concentrations potentiates the inhibition of acetylcholine release caused by morphine. In addition, the calcium channel blocker, diltiazem, was found to inhibit the release of acetylcholine. The combination of morphine and diltiazem resulted in a greater inhibition of acetylcholine release than by either alone. These results suggest that opiate cholinergic interactions occur in non-neural tissue with a mechanism similar to that known to occur at certain cholinergic synapses.",
author = "Mahmoud Ahmed and Timothy Schoof and Zhou, {De He} and Christopher Quarles",
year = "1989",
doi = "10.1016/0024-3205(89)90001-5",
language = "English (US)",
volume = "45",
pages = "2383--2393",
journal = "Life Sciences",
issn = "0024-3205",
publisher = "Elsevier Inc.",
number = "25",

}

TY - JOUR

T1 - Kappa opioid receptors of human placental villi modulate acetylcholine release

AU - Ahmed, Mahmoud

AU - Schoof, Timothy

AU - Zhou, De He

AU - Quarles, Christopher

PY - 1989

Y1 - 1989

N2 - Human placental villus tissue is non-innervated, yet it contains components of the opiate and cholinergic systems. We investigated whether opioids modulate a calcium dependent acetyltholine release from the villus tissue in a manner similar to that demonstrated by the parasympathetic nerve-smooth muscle junction. We reported that the κ receptor agonist ethylketocyclazocine (EKC) inhibits acetylcholine release, and that the inhibition is reversed by the selective antagonist, Mr2266. Findings reported here substantiate the role of opioids as modulators of acetylcholine release from villus tissue. The non-selective agonist, morphine, also inhibits acetylcholine release. Inhibition caused by morphine is reversed by low concentrations of non-selective antagonists, naloxone and naltrexone. Naloxone at high concentrations potentiates the inhibition of acetylcholine release caused by morphine. In addition, the calcium channel blocker, diltiazem, was found to inhibit the release of acetylcholine. The combination of morphine and diltiazem resulted in a greater inhibition of acetylcholine release than by either alone. These results suggest that opiate cholinergic interactions occur in non-neural tissue with a mechanism similar to that known to occur at certain cholinergic synapses.

AB - Human placental villus tissue is non-innervated, yet it contains components of the opiate and cholinergic systems. We investigated whether opioids modulate a calcium dependent acetyltholine release from the villus tissue in a manner similar to that demonstrated by the parasympathetic nerve-smooth muscle junction. We reported that the κ receptor agonist ethylketocyclazocine (EKC) inhibits acetylcholine release, and that the inhibition is reversed by the selective antagonist, Mr2266. Findings reported here substantiate the role of opioids as modulators of acetylcholine release from villus tissue. The non-selective agonist, morphine, also inhibits acetylcholine release. Inhibition caused by morphine is reversed by low concentrations of non-selective antagonists, naloxone and naltrexone. Naloxone at high concentrations potentiates the inhibition of acetylcholine release caused by morphine. In addition, the calcium channel blocker, diltiazem, was found to inhibit the release of acetylcholine. The combination of morphine and diltiazem resulted in a greater inhibition of acetylcholine release than by either alone. These results suggest that opiate cholinergic interactions occur in non-neural tissue with a mechanism similar to that known to occur at certain cholinergic synapses.

UR - http://www.scopus.com/inward/record.url?scp=0024844621&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024844621&partnerID=8YFLogxK

U2 - 10.1016/0024-3205(89)90001-5

DO - 10.1016/0024-3205(89)90001-5

M3 - Article

VL - 45

SP - 2383

EP - 2393

JO - Life Sciences

JF - Life Sciences

SN - 0024-3205

IS - 25

ER -