Keratin 2e: A marker for murine nipple epidermis

Bryon Mahler, Todd Gocken, Marija Brojan, Sue Childress, Dan F. Spandau, John Foley

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Mesenchyme-derived signals influence the unique keratinization and appendage formation programs in specialized skin regions. Interactions between primary mammary mesenchyme and epidermal cells result in the formation of the nipple; however, it is unclear whether this represents a site of regionally specialized epidermis. We profiled the ultrastructure and keratin expression of the murine nipple, and the ventral skin of the K14-parathyroid hormone-related protein (PTHrP) transgenic mouse, which models nipple formation. We found the murine nipple and ventral K14-PTHrP epidermis display expanded suprabasal and granular layers, as well as a thickened cornified layer compared to ventral skin of wild-type littermates. We also observed increased levels of filaggrin in extracts from the ventral epidermis of the K14-PTHrP mouse when compared to that of wild-type littermates. Keratin 2e, previously reported to be expressed in various specialized epidermal sites in the mouse, is expressed in the nipple and the ventral skin of the K14-PTHrP mouse. Keratinocytes grown from the ventral epidermis of the K14-PTHrP mouse or wild-type littermates exhibited identical expression of epidermal markers in vitro, suggesting that the modulated differentiation profile observed in the nipple or the ventral K14-PTHrP skin was dependent on interactions with fibroblasts. The lack of appendages, altered stratification pattern and expression of a specialized keratin suggests that the murine nipple is an example of regionally specialized epidermis.

Original languageEnglish (US)
Pages (from-to)169-177
Number of pages9
JournalCells Tissues Organs
Volume176
Issue number4
DOIs
StatePublished - 2004
Externally publishedYes

Fingerprint

Keratin-2
Nipples
Parathyroid Hormone-Related Protein
Epidermis
Skin
Mesoderm
Keratins
Keratinocytes
Transgenic Mice
Breast
Fibroblasts

Keywords

  • Keratin 2e
  • Mammary gland development
  • Mouse
  • Nipple
  • Parathyroid hormone-related protein

ASJC Scopus subject areas

  • Anatomy

Cite this

Mahler, B., Gocken, T., Brojan, M., Childress, S., Spandau, D. F., & Foley, J. (2004). Keratin 2e: A marker for murine nipple epidermis. Cells Tissues Organs, 176(4), 169-177. https://doi.org/10.1159/000077033

Keratin 2e : A marker for murine nipple epidermis. / Mahler, Bryon; Gocken, Todd; Brojan, Marija; Childress, Sue; Spandau, Dan F.; Foley, John.

In: Cells Tissues Organs, Vol. 176, No. 4, 2004, p. 169-177.

Research output: Contribution to journalArticle

Mahler, B, Gocken, T, Brojan, M, Childress, S, Spandau, DF & Foley, J 2004, 'Keratin 2e: A marker for murine nipple epidermis', Cells Tissues Organs, vol. 176, no. 4, pp. 169-177. https://doi.org/10.1159/000077033
Mahler B, Gocken T, Brojan M, Childress S, Spandau DF, Foley J. Keratin 2e: A marker for murine nipple epidermis. Cells Tissues Organs. 2004;176(4):169-177. https://doi.org/10.1159/000077033
Mahler, Bryon ; Gocken, Todd ; Brojan, Marija ; Childress, Sue ; Spandau, Dan F. ; Foley, John. / Keratin 2e : A marker for murine nipple epidermis. In: Cells Tissues Organs. 2004 ; Vol. 176, No. 4. pp. 169-177.
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