Keratinocytes are cell targets of west nile virus in vivo

Pei Yin Lim; Melissa J. Behr; Chrystal M. Chadwick; Pei Yong Shi; Kristen A. Bernard

doi:10.1128/JVI.02692-10

Keratinocytes are cell targets of west nile virus in vivo

Pei Yin Lim, Melissa J. Behr, Chrystal M. Chadwick, Pei Yong Shi, Kristen A. Bernard

Biochemistry & Molecular Biology

Research output: Contribution to journal › Article › peer-review

95 Scopus citations

Abstract

West Nile virus (WNV) replicates in the skin; however, cell targets in the skin have not been identified. In the current studies, WNV infected the epidermis and adnexal glands of mouse skin, and the epidermal cells were identified as keratinocytes by double labeling for WNV antigen and keratin 10. Inoculation of mice with WNV replicon particles resulted in high levels of replication in the skin, suggesting that keratinocytes are an initial target of WNV. In addition, primary keratinocytes produced infectious virus in vitro. In conclusion, keratinocytes are cell targets of WNV in vivo and may play an important role in pathogenesis.

Original language	English (US)
Pages (from-to)	5197-5201
Number of pages	5
Journal	Journal of virology
Volume	85
Issue number	10
DOIs	https://doi.org/10.1128/JVI.02692-10
State	Published - May 2011

ASJC Scopus subject areas

Microbiology
Immunology
Insect Science
Virology

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10.1128/JVI.02692-10

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title = "Keratinocytes are cell targets of west nile virus in vivo",

abstract = "West Nile virus (WNV) replicates in the skin; however, cell targets in the skin have not been identified. In the current studies, WNV infected the epidermis and adnexal glands of mouse skin, and the epidermal cells were identified as keratinocytes by double labeling for WNV antigen and keratin 10. Inoculation of mice with WNV replicon particles resulted in high levels of replication in the skin, suggesting that keratinocytes are an initial target of WNV. In addition, primary keratinocytes produced infectious virus in vitro. In conclusion, keratinocytes are cell targets of WNV in vivo and may play an important role in pathogenesis.",

author = "Lim, {Pei Yin} and Behr, {Melissa J.} and Chadwick, {Chrystal M.} and Shi, {Pei Yong} and Bernard, {Kristen A.}",

year = "2011",

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doi = "10.1128/JVI.02692-10",

language = "English (US)",

volume = "85",

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T1 - Keratinocytes are cell targets of west nile virus in vivo

AU - Lim, Pei Yin

AU - Behr, Melissa J.

AU - Chadwick, Chrystal M.

AU - Shi, Pei Yong

AU - Bernard, Kristen A.

PY - 2011/5

Y1 - 2011/5

N2 - West Nile virus (WNV) replicates in the skin; however, cell targets in the skin have not been identified. In the current studies, WNV infected the epidermis and adnexal glands of mouse skin, and the epidermal cells were identified as keratinocytes by double labeling for WNV antigen and keratin 10. Inoculation of mice with WNV replicon particles resulted in high levels of replication in the skin, suggesting that keratinocytes are an initial target of WNV. In addition, primary keratinocytes produced infectious virus in vitro. In conclusion, keratinocytes are cell targets of WNV in vivo and may play an important role in pathogenesis.

AB - West Nile virus (WNV) replicates in the skin; however, cell targets in the skin have not been identified. In the current studies, WNV infected the epidermis and adnexal glands of mouse skin, and the epidermal cells were identified as keratinocytes by double labeling for WNV antigen and keratin 10. Inoculation of mice with WNV replicon particles resulted in high levels of replication in the skin, suggesting that keratinocytes are an initial target of WNV. In addition, primary keratinocytes produced infectious virus in vitro. In conclusion, keratinocytes are cell targets of WNV in vivo and may play an important role in pathogenesis.

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Keratinocytes are cell targets of west nile virus in vivo

Abstract

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