Keratinocytes are cell targets of west nile virus in vivo

Pei Yin Lim, Melissa J. Behr, Chrystal M. Chadwick, Pei-Yong Shi, Kristen A. Bernard

Research output: Contribution to journalArticle

66 Scopus citations

Abstract

West Nile virus (WNV) replicates in the skin; however, cell targets in the skin have not been identified. In the current studies, WNV infected the epidermis and adnexal glands of mouse skin, and the epidermal cells were identified as keratinocytes by double labeling for WNV antigen and keratin 10. Inoculation of mice with WNV replicon particles resulted in high levels of replication in the skin, suggesting that keratinocytes are an initial target of WNV. In addition, primary keratinocytes produced infectious virus in vitro. In conclusion, keratinocytes are cell targets of WNV in vivo and may play an important role in pathogenesis.

Original languageEnglish (US)
Pages (from-to)5197-5201
Number of pages5
JournalJournal of Virology
Volume85
Issue number10
DOIs
StatePublished - May 2011
Externally publishedYes

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ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Lim, P. Y., Behr, M. J., Chadwick, C. M., Shi, P-Y., & Bernard, K. A. (2011). Keratinocytes are cell targets of west nile virus in vivo. Journal of Virology, 85(10), 5197-5201. https://doi.org/10.1128/JVI.02692-10