Kern Canyon virus

Electron microscopic and immunological studies

Frederick A. Murphy, Bernard N. Fields

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Kern Canyon virus (KCV), propagated in suckling mouse brain and cell culture, was examined by negative contrast and thin section electron microscopy. The virus was found to exhibit symmetry similar to other viruses of the Stomatoviridae family. The bullet-shaped virus particles had a mean length of 132 mμ and were 73 mμ in diameter. Cross striations, axial channels and surface projections were prominent. Virus maturation occurred on marginal cytoplasmic membranes with mature virions accumulating extracellularly. Complement fixation, virus neutralization, and immunodiffusion tests comparing KCV with other bullet-shaped viruses of animals confirmed the distinct antigenicity of the virus, whereas comparison of KCV with vesicular stomatitis virus in a density gradient ultracentrifugation experiment emphasized the physical basis for including KCV within the Stomatoviridae family of viruses.

Original languageEnglish (US)
Pages (from-to)625-637
Number of pages13
JournalVirology
Volume33
Issue number4
StatePublished - Dec 1967

Fingerprint

Electrons
Viruses
Virion
Neutralization Tests
Vesicular Stomatitis
Immunodiffusion
Ultracentrifugation
Electron Microscopy
Cell Culture Techniques
Cell Membrane
Brain

ASJC Scopus subject areas

  • Infectious Diseases
  • Virology

Cite this

Murphy, F. A., & Fields, B. N. (1967). Kern Canyon virus: Electron microscopic and immunological studies. Virology, 33(4), 625-637.

Kern Canyon virus : Electron microscopic and immunological studies. / Murphy, Frederick A.; Fields, Bernard N.

In: Virology, Vol. 33, No. 4, 12.1967, p. 625-637.

Research output: Contribution to journalArticle

Murphy, FA & Fields, BN 1967, 'Kern Canyon virus: Electron microscopic and immunological studies', Virology, vol. 33, no. 4, pp. 625-637.
Murphy FA, Fields BN. Kern Canyon virus: Electron microscopic and immunological studies. Virology. 1967 Dec;33(4):625-637.
Murphy, Frederick A. ; Fields, Bernard N. / Kern Canyon virus : Electron microscopic and immunological studies. In: Virology. 1967 ; Vol. 33, No. 4. pp. 625-637.
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