Key interactions in HIV-1 maturation identified by hydrogen-deuterium exchange

Jason Lanman, Tu Kiet T. Lam, Mark R. Emmett, Alan G. Marshall, Michael Sakalian, Peter E. Prevelige

Research output: Contribution to journalArticle

134 Scopus citations

Abstract

To characterize the intersubunit interactions underlying assembly and maturation in HIV-1, we determined the amide hydrogen exchange protection pattern of capsid protein in the immature virion and the mature virion using mass spectrometry. Alterations in protection upon maturation provide evidence for the maturation-induced formation of an interaction between the N- and C-terminal domains in half of the capsid molecules, indicating that only half of the capsid protein is assembled into the conical core.

Original languageEnglish (US)
Pages (from-to)676-677
Number of pages2
JournalNature Structural and Molecular Biology
Volume11
Issue number7
DOIs
StatePublished - Jul 1 2004
Externally publishedYes

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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