Key interactions in HIV-1 maturation identified by hydrogen-deuterium exchange

Jason Lanman, TuKiet T. Lam, Mark Emmett, Alan G. Marshall, Michael Sakalian, Peter E. Prevelige

Research output: Contribution to journalArticle

130 Citations (Scopus)

Abstract

To characterize the intersubunit interactions underlying assembly and maturation in HIV-1, we determined the amide hydrogen exchange protection pattern of capsid protein in the immature virion and the mature virion using mass spectrometry. Alterations in protection upon maturation provide evidence for the maturation-induced formation of an interaction between the N- and C-terminal domains in half of the capsid molecules, indicating that only half of the capsid protein is assembled into the conical core.

Original languageEnglish (US)
Pages (from-to)676-677
Number of pages2
JournalNature Structural and Molecular Biology
Volume11
Issue number7
DOIs
StatePublished - Jul 2004
Externally publishedYes

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Deuterium
Capsid Proteins
Virion
HIV-1
Hydrogen
Capsid
Amides
Mass Spectrometry

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

Cite this

Key interactions in HIV-1 maturation identified by hydrogen-deuterium exchange. / Lanman, Jason; Lam, TuKiet T.; Emmett, Mark; Marshall, Alan G.; Sakalian, Michael; Prevelige, Peter E.

In: Nature Structural and Molecular Biology, Vol. 11, No. 7, 07.2004, p. 676-677.

Research output: Contribution to journalArticle

Lanman, Jason ; Lam, TuKiet T. ; Emmett, Mark ; Marshall, Alan G. ; Sakalian, Michael ; Prevelige, Peter E. / Key interactions in HIV-1 maturation identified by hydrogen-deuterium exchange. In: Nature Structural and Molecular Biology. 2004 ; Vol. 11, No. 7. pp. 676-677.
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