Kinase-Independent Potentiation of B Cell Antigen Receptor-Mediated Signal Transduction by the Protein Tyrosine Kinase Src

Jiejian Lin, Jiaxian Tao, Roy B. Dyer, Norbert K. Herzog, Louis B. Justement

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Signal transduction mediated by the B cell Ag receptor involves the activation of multiple protein tyrosine kinases that are members of the Src family (i.e., Fyn, Lyn, Blk, Lck). To determine whether members of the Src family possess common physical and/or enzymatic properties that enable them to potentiate signal transduction via the B cell Ag receptor, we expressed the protein tyrosine kinase Src in the B lymphoma cell line K46-17μmλ. Based on coprecipitation analysis and two-color immunofluorescence, this heterologous Src family kinase was observed to physically associate with the B cell Ag receptor. Additional experiments demonstrated that B cell Ag receptor cross-linking results in increased tyrosine phosphorylation and activation of Src. Several parameters of B cell activation, including tyrosine phosphorylation of intracellular substrates, calcium mobilization, and transcription factor activation, were potentiated in cells that expressed Src when compared with control cells. To determine whether potentiation of Ag receptor-mediated signaling by Src was dependent on its catalytic activity, a kinase-deficient form of Src was expressed in K46-17μmλ cells. Transfectants expressing kinase-deficient Src exhibited an enhanced responsiveness to stimulation through the B cell Ag receptor that was comparable with transfectants expressing wild-type Src. Additionally, kinase-deficient Src was observed to associate with the endogenous kinase Lyn in an activation-dependent manner. These findings indicate that members of the Src family may potentiate Ag receptor-mediated signaling via a kinase-independent mechanism(s) that involves amplification of kinase recruitment to the Ag receptor activation complex.

Original languageEnglish (US)
Pages (from-to)4823-4833
Number of pages11
JournalJournal of Immunology
Volume159
Issue number10
StatePublished - Nov 15 1997

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B-Cell Antigen Receptors
src-Family Kinases
Signal Transduction
B-Lymphocytes
Phosphotransferases
Tyrosine
Phosphorylation
Receptor Protein-Tyrosine Kinases
Transcriptional Activation
Fluorescent Antibody Technique
Lymphoma
Transcription Factors
Color
Calcium
Cell Line

ASJC Scopus subject areas

  • Immunology

Cite this

Lin, J., Tao, J., Dyer, R. B., Herzog, N. K., & Justement, L. B. (1997). Kinase-Independent Potentiation of B Cell Antigen Receptor-Mediated Signal Transduction by the Protein Tyrosine Kinase Src. Journal of Immunology, 159(10), 4823-4833.

Kinase-Independent Potentiation of B Cell Antigen Receptor-Mediated Signal Transduction by the Protein Tyrosine Kinase Src. / Lin, Jiejian; Tao, Jiaxian; Dyer, Roy B.; Herzog, Norbert K.; Justement, Louis B.

In: Journal of Immunology, Vol. 159, No. 10, 15.11.1997, p. 4823-4833.

Research output: Contribution to journalArticle

Lin, J, Tao, J, Dyer, RB, Herzog, NK & Justement, LB 1997, 'Kinase-Independent Potentiation of B Cell Antigen Receptor-Mediated Signal Transduction by the Protein Tyrosine Kinase Src', Journal of Immunology, vol. 159, no. 10, pp. 4823-4833.
Lin, Jiejian ; Tao, Jiaxian ; Dyer, Roy B. ; Herzog, Norbert K. ; Justement, Louis B. / Kinase-Independent Potentiation of B Cell Antigen Receptor-Mediated Signal Transduction by the Protein Tyrosine Kinase Src. In: Journal of Immunology. 1997 ; Vol. 159, No. 10. pp. 4823-4833.
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