Kinectin, an essential anchor for kinesin-driven vesicle motility

Janardan Kumar, Hanry Yu, Michael Sheetz

Research output: Contribution to journalArticle

121 Citations (Scopus)

Abstract

The membrane anchor for the molecular motor kinesin is a critical site involved in intracellular membrane trafficking. Monoclonal antibodies specific for the cytoplasmic surface of chick brain microsomes were used to define proteins involved in microtubule-dependent transport. One of four antibodies tested inhibited plus-end-directed vesicle motility by approximately 90 percent even as a monovalent Fab fragment and reduced kinesin binding to vesicles. This antibody bound to the cytoplasmic domain of kinectin, an integral membrane protein of the endoplasmic reticulum that binds to kinesin. Thus, kinectin acted as a membrane anchor protein for kinesin-driven vesicle motility.

Original languageEnglish (US)
Pages (from-to)1834-1837
Number of pages4
JournalScience
Volume67
Issue number5205
StatePublished - Jan 1 1995
Externally publishedYes

Fingerprint

Kinesin
Membrane Proteins
Intracellular Membranes
Immunoglobulin Fab Fragments
Antibodies
Microsomes
Microtubules
Endoplasmic Reticulum
Monoclonal Antibodies
Membranes
Brain
Proteins

ASJC Scopus subject areas

  • General

Cite this

Kinectin, an essential anchor for kinesin-driven vesicle motility. / Kumar, Janardan; Yu, Hanry; Sheetz, Michael.

In: Science, Vol. 67, No. 5205, 01.01.1995, p. 1834-1837.

Research output: Contribution to journalArticle

Kumar, J, Yu, H & Sheetz, M 1995, 'Kinectin, an essential anchor for kinesin-driven vesicle motility', Science, vol. 67, no. 5205, pp. 1834-1837.
Kumar, Janardan ; Yu, Hanry ; Sheetz, Michael. / Kinectin, an essential anchor for kinesin-driven vesicle motility. In: Science. 1995 ; Vol. 67, No. 5205. pp. 1834-1837.
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