Kinectin distribution in chicken nervous system

Itaru Toyoshima, Michael Sheetz

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Kinectin, a major kinesin receptor on endoplasmic reticulum, was visualized with anti-kinectin monoclonal antibodies (mAbs) in the adult chicken nervous system in comparison with kinesin immunostaining. Anti- kinectin mAbs punctately stained cell bodies and proximal dendrites of motor neurons in spinal curds. Axons of motor neurons were not stained with anti- kinectin mAbs, but stained heavily with anti-kinesin mAbs. This suggest that the kinesin receptor responsible for kinesin-driven anterograde fast axonal transport is different from kinectin. Anti-kinectin mAbs strongly stained neuronal cell bodies in spinal ganglion, nuclei in brainstem, cerebellar nuclei, striatum and cerebral cortex. Small neurons in cerebellar cortex and optic lobe showed relatively weak reaction, suggesting that the amount of kinectin correlates with the size of neuronal cell bodies.

Original languageEnglish (US)
Pages (from-to)171-174
Number of pages4
JournalNeuroscience Letters
Volume211
Issue number3
DOIs
StatePublished - Jun 28 1996
Externally publishedYes

Fingerprint

Kinesin
Nervous System
Chickens
Monoclonal Antibodies
Cerebellar Cortex
Motor Neurons
Cerebellar Nuclei
Axonal Transport
Spinal Ganglia
Dendrites
Endoplasmic Reticulum
Cerebral Cortex
Brain Stem
Axons
Neurons
Cell Body

Keywords

  • Endoplasmic reticulum
  • Immunohistochemistry
  • Kinectin
  • Kinesin
  • Kinesin receptor
  • Molecular motor
  • Monoclonal antibody

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Kinectin distribution in chicken nervous system. / Toyoshima, Itaru; Sheetz, Michael.

In: Neuroscience Letters, Vol. 211, No. 3, 28.06.1996, p. 171-174.

Research output: Contribution to journalArticle

Toyoshima, Itaru ; Sheetz, Michael. / Kinectin distribution in chicken nervous system. In: Neuroscience Letters. 1996 ; Vol. 211, No. 3. pp. 171-174.
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