Abstract
Kinesin and cytoplasmic dynein are two major molecular motors responsible for fast axonal transport. As visualized by immunohistochemistry with monoclonal antibodies, both motors were found to be distributed throughout the cell bodies, dendrites and axons of motor neurons in normal human spinal cords. Large axonal swellings, spheroids, in the spinal cords of patients with motor neuron disease showed massive accumulation of kinesin co- localized with highly phosphorylated neurofilaments. Of 114 spheroids in five spinal cords, 87% were stained heavily with the three anti-kinesin antibodies used in this study. Cytoplasmic dynein was scarce or absent in most of the spheroids. These findings suggest that kinesin selectively accumulates in the spheroids of motor neuron axons, causing disturbance of the machinery for anterograde fast axonal transport in motor neuron disease.
Original language | English (US) |
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Pages (from-to) | 38-44 |
Number of pages | 7 |
Journal | Journal of the Neurological Sciences |
Volume | 159 |
Issue number | 1 |
DOIs | |
State | Published - Jul 15 1998 |
Externally published | Yes |
Keywords
- Amyotrophic lateral sclerosis
- Cytoplasmic dynein
- Kinesin
- Molecular motor
- Motor neuron disease
- Spheroid
ASJC Scopus subject areas
- Neurology
- Clinical Neurology