Kinetic and structural characterization of the glutathione-binding site of aldose reductase

Bharat L. Dixit, Ganesaratnam K. Balendiran, Stanley Watowich, Sanjay Srivastava, Kota Ramana, J. Mark Petrash, Aruni Bhatnagar, Satish Srivastava

Research output: Contribution to journalArticle

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Abstract

Aldose reductase (AR), a member of the aldo-keto reductase superfamily, has been implicated in the etiology of secondary diabetic complications. However, the physiological functions of AR under euglycemic conditions remain unclear. We have recently demonstrated that, in intact heart, AR catalyzes the reduction of the glutathione conjugate of the lipid peroxidation product 4-hydroxy-trans-2-nonenal (Srivastava, S., Chandra, A., Wang, L., Seifert, W. E., Jr., DaGue, B. B., Ansari, N. H., Srivastava, S. K., and Bhatnagar, A. (1998) J. Biol. Chem. 273, 10893-10900), consistent with a possible role of AR in the metabolism of glutathione conjugates of aldehydes. Herein, we present several lines of evidence suggesting that the active site of AR forms a specific glutathione-binding domain. The catalytic efficiency of AR in the reduction of the glutathione conjugates of acrolein, trans-2-hexenal, trans- 2-nonenal, and trans-trans-2,4-decadienal was 4-1000-fold higher than for the corresponding free alkanal. Alterations in the structure of glutathione diminished the catalytic efficiency in the reduction of the acrolein adduct, consistent with the presence of specific interactions between the amino acid residues of glutathione and the AR active site. In addition, non-aldehydic conjugates of glutathione or glutathione analogs displayed active-site inhibition. Molecular dynamics calculations suggest that the conjugate adopts a specific low energy configuration at the active site, indicating selective binding. These observations support an important role of AR in the metabolism of glutathione conjugates of endogenous and xenobiotic aldehydes and demonstrate, for the first time, efficient binding of glutathione conjugates to an aldo-keto reductase.

Original languageEnglish (US)
Pages (from-to)21587-21595
Number of pages9
JournalJournal of Biological Chemistry
Volume275
Issue number28
DOIs
StatePublished - Jul 14 2000

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Aldehyde Reductase
Glutathione
Binding Sites
Kinetics
Catalytic Domain
Acrolein
Aldehydes
Metabolism
Glutathione Reductase
Xenobiotics
Diabetes Complications
Molecular Dynamics Simulation
Lipid Peroxidation
Molecular dynamics
Amino Acids
Lipids

ASJC Scopus subject areas

  • Biochemistry

Cite this

Kinetic and structural characterization of the glutathione-binding site of aldose reductase. / Dixit, Bharat L.; Balendiran, Ganesaratnam K.; Watowich, Stanley; Srivastava, Sanjay; Ramana, Kota; Petrash, J. Mark; Bhatnagar, Aruni; Srivastava, Satish.

In: Journal of Biological Chemistry, Vol. 275, No. 28, 14.07.2000, p. 21587-21595.

Research output: Contribution to journalArticle

Dixit, BL, Balendiran, GK, Watowich, S, Srivastava, S, Ramana, K, Petrash, JM, Bhatnagar, A & Srivastava, S 2000, 'Kinetic and structural characterization of the glutathione-binding site of aldose reductase', Journal of Biological Chemistry, vol. 275, no. 28, pp. 21587-21595. https://doi.org/10.1074/jbc.M909235199
Dixit, Bharat L. ; Balendiran, Ganesaratnam K. ; Watowich, Stanley ; Srivastava, Sanjay ; Ramana, Kota ; Petrash, J. Mark ; Bhatnagar, Aruni ; Srivastava, Satish. / Kinetic and structural characterization of the glutathione-binding site of aldose reductase. In: Journal of Biological Chemistry. 2000 ; Vol. 275, No. 28. pp. 21587-21595.
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abstract = "Aldose reductase (AR), a member of the aldo-keto reductase superfamily, has been implicated in the etiology of secondary diabetic complications. However, the physiological functions of AR under euglycemic conditions remain unclear. We have recently demonstrated that, in intact heart, AR catalyzes the reduction of the glutathione conjugate of the lipid peroxidation product 4-hydroxy-trans-2-nonenal (Srivastava, S., Chandra, A., Wang, L., Seifert, W. E., Jr., DaGue, B. B., Ansari, N. H., Srivastava, S. K., and Bhatnagar, A. (1998) J. Biol. Chem. 273, 10893-10900), consistent with a possible role of AR in the metabolism of glutathione conjugates of aldehydes. Herein, we present several lines of evidence suggesting that the active site of AR forms a specific glutathione-binding domain. The catalytic efficiency of AR in the reduction of the glutathione conjugates of acrolein, trans-2-hexenal, trans- 2-nonenal, and trans-trans-2,4-decadienal was 4-1000-fold higher than for the corresponding free alkanal. Alterations in the structure of glutathione diminished the catalytic efficiency in the reduction of the acrolein adduct, consistent with the presence of specific interactions between the amino acid residues of glutathione and the AR active site. In addition, non-aldehydic conjugates of glutathione or glutathione analogs displayed active-site inhibition. Molecular dynamics calculations suggest that the conjugate adopts a specific low energy configuration at the active site, indicating selective binding. These observations support an important role of AR in the metabolism of glutathione conjugates of endogenous and xenobiotic aldehydes and demonstrate, for the first time, efficient binding of glutathione conjugates to an aldo-keto reductase.",
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AU - Balendiran, Ganesaratnam K.

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AU - Srivastava, Sanjay

AU - Ramana, Kota

AU - Petrash, J. Mark

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AU - Srivastava, Satish

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