L-arginine decreases fluid-percussion injury-induced neuronal nitrotyrosine immunoreactivity in rats

Marcela A. Avila, Stacy Sell, Yuji Kadoi, Donald Prough, Helen Hellmich, Marco Velasco, Douglas Dewitt

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Peroxynitrite is a powerful oxidant capable of nitrating phenolic moieties, such as tyrosine or tyrosine residues in proteins and increases after traumatic brain injury (TBI). First, we tested the hypothesis that TBI increases nitrotyrosine (NT) immunoreactivity in the brain by measuring the number of NT-immunoreactive neurons in the cerebral cortex and hippocampus of rats subjected to parasagittal fluid-percussion TBI. Second, we tested the hypothesis that treatment with L-arginine, a substrate for nitric oxide synthase, further increases NT immunoreactivity over TBI alone. Rats were anesthetized with isoflurane and subjected to TBI, sham TBI, or TBI followed by treatment with L-arginine (100 mg/kg). Twelve, 24, or 72 h after TBI, brains were harvested. Coronal sections (10 μm) were incubated overnight with rabbit polyclonal anti-NT antibody, rinsed, and incubated with a biotinylated secondary antibody. The antigen-antibody complex was visualized using a peroxidase-conjugated system with diaminobenzidine as the chromagen. The number of NT-positive cortical and hippocampal neurons increased significantly in both ipsilateral and contralateral hemispheres up to 72 h after TBI compared with the sham-injured group. Remarkably, treatment with L-arginine reduced the number of NT-positive neurons after TBI in both cortex and hippocampus. Our results indicate that L-arginine actually prevents TBI-induced increases in NT immunoreactivity.

Original languageEnglish (US)
Pages (from-to)1733-1741
Number of pages9
JournalJournal of Cerebral Blood Flow and Metabolism
Volume28
Issue number10
DOIs
StatePublished - Oct 2008

Fingerprint

Percussion
Arginine
Wounds and Injuries
Neurons
Tyrosine
Hippocampus
3-nitrotyrosine
Traumatic Brain Injury
Peroxynitrous Acid
Isoflurane
Brain
Antigen-Antibody Complex
Oxidants
Nitric Oxide Synthase
Cerebral Cortex
Peroxidase
Anti-Idiotypic Antibodies

Keywords

  • Immunoreactivity
  • L-arginine
  • Nitrotyrosine
  • Rats
  • Traumatic brain injury

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Clinical Neurology
  • Neurology

Cite this

L-arginine decreases fluid-percussion injury-induced neuronal nitrotyrosine immunoreactivity in rats. / Avila, Marcela A.; Sell, Stacy; Kadoi, Yuji; Prough, Donald; Hellmich, Helen; Velasco, Marco; Dewitt, Douglas.

In: Journal of Cerebral Blood Flow and Metabolism, Vol. 28, No. 10, 10.2008, p. 1733-1741.

Research output: Contribution to journalArticle

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