Laboratory diagnosis of Rocky Mountain spotted fever by immunofluorescent demonstration of Rickettsia in Cutaneous lesions.

D. H. Walker, B. G. Cain, P. M. Olmstead

Research output: Contribution to journalArticle

Abstract

Direct immunofluorescent staining for Rickettsia rickettsii was performed on cryostat sections of skin biopsies from 27 patients suspected of having Rocky Mountain spotted fever. In nine of the 17 patients whose final diagnosis was Rocky Mountain spotted fever, coccobacillary forms of R. rickettsii were identified in endothelium and vascular walls within the dermis. Facotrs recognized as contributing to false-negative results were prior treatment with tetracycline or chloramphenicol for 24--48 hours or longer and failure to obtain a section through the focus of vasculitis. No false-positive result was obtained in the ten patients whose final diagnoses were not Rocky Mountain spotted fever. The laboratory test offers an immediate, positive laboratory diagnosis for this treatable, life-threatening disease.

Original languageEnglish (US)
Pages (from-to)619-623
Number of pages5
JournalAmerican Journal of Clinical Pathology
Volume69
Issue number6
StatePublished - Jun 1978
Externally publishedYes

Fingerprint

Rocky Mountain Spotted Fever
Rickettsia
Clinical Laboratory Techniques
Rickettsia rickettsii
Skin
Vascular Endothelium
Chloramphenicol
Dermis
Vasculitis
Tetracycline
Staining and Labeling
Biopsy
Therapeutics

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Laboratory diagnosis of Rocky Mountain spotted fever by immunofluorescent demonstration of Rickettsia in Cutaneous lesions. / Walker, D. H.; Cain, B. G.; Olmstead, P. M.

In: American Journal of Clinical Pathology, Vol. 69, No. 6, 06.1978, p. 619-623.

Research output: Contribution to journalArticle

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