Laboratory studies of transovarial transmission of La Crosse and other arboviruses by Aedes albopictus and Culex fatigans

R. B. Tesh, D. J. Gubler

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Transovarial transmission of La Crosse virus by experimentally infected Aedes albopictus females to 2.7% of their F1 generation offspring was demonstrated. Progeny of both sexes were infected. Mean virus titers in parent mosquitoes and infected F1 generation adults were 10(superior 4.6) and 10(superior 3.4) plaque forming units/insect, respectively. The La Crosse infected offspring were randomly distributed among the female parents. After 2 serial passages in A. albopictus, a marked change occurred in the plaque morphology of the virus but this had no apparent effect on the subsequent vertical transmission rate. In contrast, transovarial transmission did not occur in La Crosse infected Culex fatigans or in A. albopictus and C. fatigans infected with vesicular stomatitis Indiana, Cache Valley, Batai, Arumowot, and Itaporanga viruses. Results of this experiment suggest that the La Crosse model might be useful in studying the mechanism of transovarial transmission in additional mosquito species.

Original languageEnglish (US)
Pages (from-to)876-880
Number of pages5
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume24
Issue number5
StatePublished - 1975
Externally publishedYes

Fingerprint

Arboviruses
Culex
Aedes
Culicidae
La Crosse virus
Viruses
Serial Passage
Vesicular Stomatitis
Viral Load
Insects

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

Laboratory studies of transovarial transmission of La Crosse and other arboviruses by Aedes albopictus and Culex fatigans. / Tesh, R. B.; Gubler, D. J.

In: American Journal of Tropical Medicine and Hygiene, Vol. 24, No. 5, 1975, p. 876-880.

Research output: Contribution to journalArticle

@article{ebfe884797914da9ad992f96d66bba61,
title = "Laboratory studies of transovarial transmission of La Crosse and other arboviruses by Aedes albopictus and Culex fatigans",
abstract = "Transovarial transmission of La Crosse virus by experimentally infected Aedes albopictus females to 2.7{\%} of their F1 generation offspring was demonstrated. Progeny of both sexes were infected. Mean virus titers in parent mosquitoes and infected F1 generation adults were 10(superior 4.6) and 10(superior 3.4) plaque forming units/insect, respectively. The La Crosse infected offspring were randomly distributed among the female parents. After 2 serial passages in A. albopictus, a marked change occurred in the plaque morphology of the virus but this had no apparent effect on the subsequent vertical transmission rate. In contrast, transovarial transmission did not occur in La Crosse infected Culex fatigans or in A. albopictus and C. fatigans infected with vesicular stomatitis Indiana, Cache Valley, Batai, Arumowot, and Itaporanga viruses. Results of this experiment suggest that the La Crosse model might be useful in studying the mechanism of transovarial transmission in additional mosquito species.",
author = "Tesh, {R. B.} and Gubler, {D. J.}",
year = "1975",
language = "English (US)",
volume = "24",
pages = "876--880",
journal = "American Journal of Tropical Medicine and Hygiene",
issn = "0002-9637",
publisher = "American Society of Tropical Medicine and Hygiene",
number = "5",

}

TY - JOUR

T1 - Laboratory studies of transovarial transmission of La Crosse and other arboviruses by Aedes albopictus and Culex fatigans

AU - Tesh, R. B.

AU - Gubler, D. J.

PY - 1975

Y1 - 1975

N2 - Transovarial transmission of La Crosse virus by experimentally infected Aedes albopictus females to 2.7% of their F1 generation offspring was demonstrated. Progeny of both sexes were infected. Mean virus titers in parent mosquitoes and infected F1 generation adults were 10(superior 4.6) and 10(superior 3.4) plaque forming units/insect, respectively. The La Crosse infected offspring were randomly distributed among the female parents. After 2 serial passages in A. albopictus, a marked change occurred in the plaque morphology of the virus but this had no apparent effect on the subsequent vertical transmission rate. In contrast, transovarial transmission did not occur in La Crosse infected Culex fatigans or in A. albopictus and C. fatigans infected with vesicular stomatitis Indiana, Cache Valley, Batai, Arumowot, and Itaporanga viruses. Results of this experiment suggest that the La Crosse model might be useful in studying the mechanism of transovarial transmission in additional mosquito species.

AB - Transovarial transmission of La Crosse virus by experimentally infected Aedes albopictus females to 2.7% of their F1 generation offspring was demonstrated. Progeny of both sexes were infected. Mean virus titers in parent mosquitoes and infected F1 generation adults were 10(superior 4.6) and 10(superior 3.4) plaque forming units/insect, respectively. The La Crosse infected offspring were randomly distributed among the female parents. After 2 serial passages in A. albopictus, a marked change occurred in the plaque morphology of the virus but this had no apparent effect on the subsequent vertical transmission rate. In contrast, transovarial transmission did not occur in La Crosse infected Culex fatigans or in A. albopictus and C. fatigans infected with vesicular stomatitis Indiana, Cache Valley, Batai, Arumowot, and Itaporanga viruses. Results of this experiment suggest that the La Crosse model might be useful in studying the mechanism of transovarial transmission in additional mosquito species.

UR - http://www.scopus.com/inward/record.url?scp=0016745966&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0016745966&partnerID=8YFLogxK

M3 - Article

C2 - 1190373

AN - SCOPUS:0016745966

VL - 24

SP - 876

EP - 880

JO - American Journal of Tropical Medicine and Hygiene

JF - American Journal of Tropical Medicine and Hygiene

SN - 0002-9637

IS - 5

ER -