Laboratory vector competence of Culex (Melanoconion) cedecei for sympatric and allopatric Venezuelan equine encephalomyelitis viruses

Scott Weaver, W. F. Scherer, C. A. Taylor, D. A. Castello, E. W. Cupp

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Laboratory vector competence of Culex (Melanoconion) cedecei was examined for Venezuelan equine encephalomyelitis (VEE) viruses. Colonized adult female mosquitoes originating from a southern Florida population were given bloodmeals from viremic hamsters circulating various titers of 3 hemagglutination inhibition (HI) subtypes of VEE viruses. Following extrinsic incubation of about 3 weeks, mosquitoes were allowed to refeed on uninfected hamsters for transmission trials. Cx. cedecei was highly efficient in becoming infected with and transmitting its sympatric, HI subtype II 'Everglades' virus. With bloodmeal titers of 100.9 chick embryo cell culture (CEC) plaque forming units (PFU), the infection rate was 9% and transmission occurred following extrinsic incubation. Infection rates were ≥80% with oral doses of ≥101.8, and all infected mosquitoes were capable of transmission following incubation. Cx. cedecei was also highly sensitive to infection with allopatric HI subtype IE Middle American VEE virus isolates. Infection rates were ≥50% with bloodmeal titers undetectable by CEC assay. Rates were 100% with oral doses of ≥100.8 CECPFU. Transmission rates were 100% in all experiments. Similar results were obtained with HI subtype IAB 'epizootic' VEE virus isolates from the 1969 Middle American outbreak. Infection rates were 100% with oral doses of ≥101.2, and transmission rates were 100% after extrinsic incubation. Comparisons with laboratory vector competence of the Middle American enzootic VEE virus vector, Culex (Melanoconion) taeniopus, are discussed.

Original languageEnglish (US)
Pages (from-to)619-623
Number of pages5
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume35
Issue number3
StatePublished - 1986
Externally publishedYes

Fingerprint

Venezuelan Equine Encephalitis Viruses
Culex
Mental Competency
Hemagglutination
Culicidae
Infection
Cricetinae
Cell Culture Techniques
Chick Embryo
Disease Outbreaks
Viruses
Inhibition (Psychology)
Population

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

Laboratory vector competence of Culex (Melanoconion) cedecei for sympatric and allopatric Venezuelan equine encephalomyelitis viruses. / Weaver, Scott; Scherer, W. F.; Taylor, C. A.; Castello, D. A.; Cupp, E. W.

In: American Journal of Tropical Medicine and Hygiene, Vol. 35, No. 3, 1986, p. 619-623.

Research output: Contribution to journalArticle

@article{34cab65a9f754412935a5176a63b911b,
title = "Laboratory vector competence of Culex (Melanoconion) cedecei for sympatric and allopatric Venezuelan equine encephalomyelitis viruses",
abstract = "Laboratory vector competence of Culex (Melanoconion) cedecei was examined for Venezuelan equine encephalomyelitis (VEE) viruses. Colonized adult female mosquitoes originating from a southern Florida population were given bloodmeals from viremic hamsters circulating various titers of 3 hemagglutination inhibition (HI) subtypes of VEE viruses. Following extrinsic incubation of about 3 weeks, mosquitoes were allowed to refeed on uninfected hamsters for transmission trials. Cx. cedecei was highly efficient in becoming infected with and transmitting its sympatric, HI subtype II 'Everglades' virus. With bloodmeal titers of 100.9 chick embryo cell culture (CEC) plaque forming units (PFU), the infection rate was 9{\%} and transmission occurred following extrinsic incubation. Infection rates were ≥80{\%} with oral doses of ≥101.8, and all infected mosquitoes were capable of transmission following incubation. Cx. cedecei was also highly sensitive to infection with allopatric HI subtype IE Middle American VEE virus isolates. Infection rates were ≥50{\%} with bloodmeal titers undetectable by CEC assay. Rates were 100{\%} with oral doses of ≥100.8 CECPFU. Transmission rates were 100{\%} in all experiments. Similar results were obtained with HI subtype IAB 'epizootic' VEE virus isolates from the 1969 Middle American outbreak. Infection rates were 100{\%} with oral doses of ≥101.2, and transmission rates were 100{\%} after extrinsic incubation. Comparisons with laboratory vector competence of the Middle American enzootic VEE virus vector, Culex (Melanoconion) taeniopus, are discussed.",
author = "Scott Weaver and Scherer, {W. F.} and Taylor, {C. A.} and Castello, {D. A.} and Cupp, {E. W.}",
year = "1986",
language = "English (US)",
volume = "35",
pages = "619--623",
journal = "American Journal of Tropical Medicine and Hygiene",
issn = "0002-9637",
publisher = "American Society of Tropical Medicine and Hygiene",
number = "3",

}

TY - JOUR

T1 - Laboratory vector competence of Culex (Melanoconion) cedecei for sympatric and allopatric Venezuelan equine encephalomyelitis viruses

AU - Weaver, Scott

AU - Scherer, W. F.

AU - Taylor, C. A.

AU - Castello, D. A.

AU - Cupp, E. W.

PY - 1986

Y1 - 1986

N2 - Laboratory vector competence of Culex (Melanoconion) cedecei was examined for Venezuelan equine encephalomyelitis (VEE) viruses. Colonized adult female mosquitoes originating from a southern Florida population were given bloodmeals from viremic hamsters circulating various titers of 3 hemagglutination inhibition (HI) subtypes of VEE viruses. Following extrinsic incubation of about 3 weeks, mosquitoes were allowed to refeed on uninfected hamsters for transmission trials. Cx. cedecei was highly efficient in becoming infected with and transmitting its sympatric, HI subtype II 'Everglades' virus. With bloodmeal titers of 100.9 chick embryo cell culture (CEC) plaque forming units (PFU), the infection rate was 9% and transmission occurred following extrinsic incubation. Infection rates were ≥80% with oral doses of ≥101.8, and all infected mosquitoes were capable of transmission following incubation. Cx. cedecei was also highly sensitive to infection with allopatric HI subtype IE Middle American VEE virus isolates. Infection rates were ≥50% with bloodmeal titers undetectable by CEC assay. Rates were 100% with oral doses of ≥100.8 CECPFU. Transmission rates were 100% in all experiments. Similar results were obtained with HI subtype IAB 'epizootic' VEE virus isolates from the 1969 Middle American outbreak. Infection rates were 100% with oral doses of ≥101.2, and transmission rates were 100% after extrinsic incubation. Comparisons with laboratory vector competence of the Middle American enzootic VEE virus vector, Culex (Melanoconion) taeniopus, are discussed.

AB - Laboratory vector competence of Culex (Melanoconion) cedecei was examined for Venezuelan equine encephalomyelitis (VEE) viruses. Colonized adult female mosquitoes originating from a southern Florida population were given bloodmeals from viremic hamsters circulating various titers of 3 hemagglutination inhibition (HI) subtypes of VEE viruses. Following extrinsic incubation of about 3 weeks, mosquitoes were allowed to refeed on uninfected hamsters for transmission trials. Cx. cedecei was highly efficient in becoming infected with and transmitting its sympatric, HI subtype II 'Everglades' virus. With bloodmeal titers of 100.9 chick embryo cell culture (CEC) plaque forming units (PFU), the infection rate was 9% and transmission occurred following extrinsic incubation. Infection rates were ≥80% with oral doses of ≥101.8, and all infected mosquitoes were capable of transmission following incubation. Cx. cedecei was also highly sensitive to infection with allopatric HI subtype IE Middle American VEE virus isolates. Infection rates were ≥50% with bloodmeal titers undetectable by CEC assay. Rates were 100% with oral doses of ≥100.8 CECPFU. Transmission rates were 100% in all experiments. Similar results were obtained with HI subtype IAB 'epizootic' VEE virus isolates from the 1969 Middle American outbreak. Infection rates were 100% with oral doses of ≥101.2, and transmission rates were 100% after extrinsic incubation. Comparisons with laboratory vector competence of the Middle American enzootic VEE virus vector, Culex (Melanoconion) taeniopus, are discussed.

UR - http://www.scopus.com/inward/record.url?scp=0022588713&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022588713&partnerID=8YFLogxK

M3 - Article

VL - 35

SP - 619

EP - 623

JO - American Journal of Tropical Medicine and Hygiene

JF - American Journal of Tropical Medicine and Hygiene

SN - 0002-9637

IS - 3

ER -