Lack of differential g2 chromosomal radiosensitivity between non-tumorigenic and tumorigenic human hybrid cells (hela × skin fibroblasts)

J. L. Redpath, C. Sun, M. Colman, Bradford Loucas, M. Muhllman-Diaz, J. S. Bedford

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Previous studies have shown, that for log phase cultures, tumorigenic segregants of HeLa × skin fibroblast human hybrid cells are slightly more radiosensitive in terms of cell killing than their nontumorigenic parents (Redpath et al. 1985, Colman et al. 1988). Other studies have shown that these same tumorigenic segregants exhibit a markedly enhanced G2 chromosomal radiosensitivity (Sanford et al. 1986) thus offering a possible explanation for the cell killing data. The present study set out to examine the G2-phase radiosensitivity of these cells in terms of cell killing with the expectation that an enhanced sensitivity in the tumorigenic cells would be seen. No enhanced sensitivity was observed. The G2 chromosomal radiosensitivity was then examined and no differential was seen between the non-tumorigenic and tumorigenic cells. This lack of confirmation of previously reported studies may be due to some technical differences in the experimental protocols.

Original languageEnglish (US)
Pages (from-to)679-685
Number of pages7
JournalInternational Journal of Radiation Biology
Volume62
Issue number6
DOIs
StatePublished - 1992
Externally publishedYes

Fingerprint

Hybrid Cells
Radiation Tolerance
fibroblasts
radiation tolerance
Fibroblasts
skin (animal)
Skin
Cells
cells
sensitivity
Cell culture
G2 Phase
interphase

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology
  • Agricultural and Biological Sciences (miscellaneous)
  • Nuclear Energy and Engineering
  • Radiation

Cite this

Lack of differential g2 chromosomal radiosensitivity between non-tumorigenic and tumorigenic human hybrid cells (hela × skin fibroblasts). / Redpath, J. L.; Sun, C.; Colman, M.; Loucas, Bradford; Muhllman-Diaz, M.; Bedford, J. S.

In: International Journal of Radiation Biology, Vol. 62, No. 6, 1992, p. 679-685.

Research output: Contribution to journalArticle

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AU - Loucas, Bradford

AU - Muhllman-Diaz, M.

AU - Bedford, J. S.

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