Lack of long-term durability of cultured keratinocyte burn-wound coverage: A case report

Manubhai H. Desai, Joseph M. Mlakar, Robert L. McCauley, Kay M. Abdullah, Randi L. Rutan, J. Paul Waymack, Martin C. Robson, David N. Herndon

Research output: Contribution to journalArticle

108 Scopus citations

Abstract

Cultured epithelial autografts have been advocated for permanent closure of skin surfaces after massive thermal injuries. A 10-year-old boy sustained a nearly 100% total body surface area burn (98% full-thickness) in an explosion accident. Cultured epithelial autografi: Was used to cover 70% of the total body surface area on postburn day 26. In spite of early success of coverage, 60% of cultured epithelial autografi: Areas blistered and sloughed over the ensuing weeks. Electron microscopic examination of a biopsy specimen of the healed cultured epithelial autografi: (80 days after placement) revealed a lack of dermal attachments of the anchoring fibrils. Additionally, blister fluid that was taken from the bullae of the cultured epidielial autografi: Revealed levels of 18 ng/ml thromboxane and 24 ng/ml prostaglandin E2. These levels are significantly higher dian those seen in acute burn blister fluid and indicate an ongoing inflammatory process. Cultured keratinocytes, although they provide early wound closure, may not provide adequate long-term coverage for patients widi massive burns.

Original languageEnglish (US)
Pages (from-to)540-545
Number of pages6
JournalJournal of Burn Care and Rehabilitation
Volume12
Issue number6
DOIs
StatePublished - Nov 1991

ASJC Scopus subject areas

  • Surgery
  • Nursing(all)
  • Emergency Medicine
  • Rehabilitation
  • Health Professions(all)

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    Desai, M. H., Mlakar, J. M., McCauley, R. L., Abdullah, K. M., Rutan, R. L., Paul Waymack, J., Robson, M. C., & Herndon, D. N. (1991). Lack of long-term durability of cultured keratinocyte burn-wound coverage: A case report. Journal of Burn Care and Rehabilitation, 12(6), 540-545. https://doi.org/10.1097/00004630-199111000-00009