Lack of long-term durability of cultured keratinocyte burn-wound coverage

A case report

M. H. Desai, J. M. Mlakar, R. L. McCauley, K. M. Abdullah, R. L. Rutan, J. P. Waymack, M. C. Robson, David Herndon

Research output: Contribution to journalArticle

103 Citations (Scopus)

Abstract

Cultured epithelial autografts have been advocated for permanent closure of skin surfaces after massive thermal injuries. A 10-year-old boy sustained a nearly 100% total body surface area burn (98% full-thickness) in an explosion accident. Cultured epithelial autograft was used to cover 70% of the total body surface area on postburn day 26. In spite of early success of coverage, 60% of cultured epithelial autograft areas blistered and sloughed over the ensuing weeks. Electron microscopic examination of a biopsy specimen of the healed cultured epithelial autograft (80 days after placement) revealed a lack of dermal attachments of the anchoring fibrils. Additionally, blister fluid that was taken from the bullae of the cultured epithelial autograft revealed levels of 18 ng/ml thromboxane and 24 ng/ml prostaglandin E2. These levels are significantly higher than those seen in acute burn blister fluid and indicate an ongoing inflammatory process. Cultured keratinocytes, although they provide early wound closure, may not provide adequate long-term coverage for patients with massive burns.

Original languageEnglish (US)
Pages (from-to)540-545
Number of pages6
JournalJournal of Burn Care and Rehabilitation
Volume12
Issue number6
StatePublished - 1991

Fingerprint

Autografts
Burns
Keratinocytes
Blister
Wounds and Injuries
Body Surface Area
Skin
Explosions
Thromboxanes
Dinoprostone
Accidents
Hot Temperature
Electrons
Biopsy

ASJC Scopus subject areas

  • Emergency Medicine
  • Rehabilitation
  • Surgery
  • Nursing(all)
  • Health Professions(all)

Cite this

Desai, M. H., Mlakar, J. M., McCauley, R. L., Abdullah, K. M., Rutan, R. L., Waymack, J. P., ... Herndon, D. (1991). Lack of long-term durability of cultured keratinocyte burn-wound coverage: A case report. Journal of Burn Care and Rehabilitation, 12(6), 540-545.

Lack of long-term durability of cultured keratinocyte burn-wound coverage : A case report. / Desai, M. H.; Mlakar, J. M.; McCauley, R. L.; Abdullah, K. M.; Rutan, R. L.; Waymack, J. P.; Robson, M. C.; Herndon, David.

In: Journal of Burn Care and Rehabilitation, Vol. 12, No. 6, 1991, p. 540-545.

Research output: Contribution to journalArticle

Desai, MH, Mlakar, JM, McCauley, RL, Abdullah, KM, Rutan, RL, Waymack, JP, Robson, MC & Herndon, D 1991, 'Lack of long-term durability of cultured keratinocyte burn-wound coverage: A case report', Journal of Burn Care and Rehabilitation, vol. 12, no. 6, pp. 540-545.
Desai MH, Mlakar JM, McCauley RL, Abdullah KM, Rutan RL, Waymack JP et al. Lack of long-term durability of cultured keratinocyte burn-wound coverage: A case report. Journal of Burn Care and Rehabilitation. 1991;12(6):540-545.
Desai, M. H. ; Mlakar, J. M. ; McCauley, R. L. ; Abdullah, K. M. ; Rutan, R. L. ; Waymack, J. P. ; Robson, M. C. ; Herndon, David. / Lack of long-term durability of cultured keratinocyte burn-wound coverage : A case report. In: Journal of Burn Care and Rehabilitation. 1991 ; Vol. 12, No. 6. pp. 540-545.
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