Lack of significant morphological differences between human X and Y spermatozoa and their precursor cells (spermatids) exposed to different prehybridization treatments

Amjad Hossain, S. Barik, P. M. Kulkarni

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Human X and Y spermatozoa were previously compared by several nonmolecular techniques. Recent studies show that in many of the previous investigations, the methods used to identify the spermatozoa were nonspecific and thus produced contradictory findings. In the present study, the comparison of the 2 germ cell types, X and Y, were performed following fluorescence in situ hybridization (FISH), which is the most reliable genotyping technique currently available. The FISH technique was performed under 3 different treatments: permeabilization with liquid N2, fixation with Carnoy's, and chromatin decondensation with lithium di-iodosalicylate. Mature and immature germ cells (spermatozoa and spermatids) were compared. Lithium showed higher hybridization efficiency, while liquid N2 and Carnoy's fixative maintained better morphological integrity of cells with lower hybridization. The sperm exhibiting hybridization signals were not different in any of the morphometric or qualitative comparisons from those that did not exhibit signals. No significant deviation of the sex ratio from 1:1 was seen in either the mature or immature germ cell population. The spatial distribution of X and Y chromosome-specific signals in the sperm head were identical. The hybridization treatments did not have any preferential effect on the cells of specific genotype (X or Y). Neither head parameters (length, HL; width, HW; area, HA) nor tail length (TL) significantly differed between X and Y populations of spermatozoa under any of the treatments. Similarly, the haploid, X-specific round cells did not differ from Y-specific ones by their size (diameter) and shape. These results indicate that neither mature sperm nor their precursors possess significant morphological differences between X and Y genotypes.

Original languageEnglish (US)
Pages (from-to)119-123
Number of pages5
JournalJournal of Andrology
Volume22
Issue number1
StatePublished - 2001
Externally publishedYes

Fingerprint

Spermatids
Spermatozoa
Germ Cells
Fluorescence In Situ Hybridization
Genotyping Techniques
Genotype
Sperm Head
Fixatives
Nitrogen Fixation
Y Chromosome
Haploidy
Sex Ratio
X Chromosome
Lithium
Population
Chromatin
Tail
Head

Keywords

  • FISH technique
  • Morphological comparison
  • Spermatid

ASJC Scopus subject areas

  • Endocrinology

Cite this

@article{72adcd2e36a44850b5ffe7405fe96cd3,
title = "Lack of significant morphological differences between human X and Y spermatozoa and their precursor cells (spermatids) exposed to different prehybridization treatments",
abstract = "Human X and Y spermatozoa were previously compared by several nonmolecular techniques. Recent studies show that in many of the previous investigations, the methods used to identify the spermatozoa were nonspecific and thus produced contradictory findings. In the present study, the comparison of the 2 germ cell types, X and Y, were performed following fluorescence in situ hybridization (FISH), which is the most reliable genotyping technique currently available. The FISH technique was performed under 3 different treatments: permeabilization with liquid N2, fixation with Carnoy's, and chromatin decondensation with lithium di-iodosalicylate. Mature and immature germ cells (spermatozoa and spermatids) were compared. Lithium showed higher hybridization efficiency, while liquid N2 and Carnoy's fixative maintained better morphological integrity of cells with lower hybridization. The sperm exhibiting hybridization signals were not different in any of the morphometric or qualitative comparisons from those that did not exhibit signals. No significant deviation of the sex ratio from 1:1 was seen in either the mature or immature germ cell population. The spatial distribution of X and Y chromosome-specific signals in the sperm head were identical. The hybridization treatments did not have any preferential effect on the cells of specific genotype (X or Y). Neither head parameters (length, HL; width, HW; area, HA) nor tail length (TL) significantly differed between X and Y populations of spermatozoa under any of the treatments. Similarly, the haploid, X-specific round cells did not differ from Y-specific ones by their size (diameter) and shape. These results indicate that neither mature sperm nor their precursors possess significant morphological differences between X and Y genotypes.",
keywords = "FISH technique, Morphological comparison, Spermatid",
author = "Amjad Hossain and S. Barik and Kulkarni, {P. M.}",
year = "2001",
language = "English (US)",
volume = "22",
pages = "119--123",
journal = "Journal of Andrology",
issn = "0196-3635",
publisher = "American Society of Andrology Inc.",
number = "1",

}

TY - JOUR

T1 - Lack of significant morphological differences between human X and Y spermatozoa and their precursor cells (spermatids) exposed to different prehybridization treatments

AU - Hossain, Amjad

AU - Barik, S.

AU - Kulkarni, P. M.

PY - 2001

Y1 - 2001

N2 - Human X and Y spermatozoa were previously compared by several nonmolecular techniques. Recent studies show that in many of the previous investigations, the methods used to identify the spermatozoa were nonspecific and thus produced contradictory findings. In the present study, the comparison of the 2 germ cell types, X and Y, were performed following fluorescence in situ hybridization (FISH), which is the most reliable genotyping technique currently available. The FISH technique was performed under 3 different treatments: permeabilization with liquid N2, fixation with Carnoy's, and chromatin decondensation with lithium di-iodosalicylate. Mature and immature germ cells (spermatozoa and spermatids) were compared. Lithium showed higher hybridization efficiency, while liquid N2 and Carnoy's fixative maintained better morphological integrity of cells with lower hybridization. The sperm exhibiting hybridization signals were not different in any of the morphometric or qualitative comparisons from those that did not exhibit signals. No significant deviation of the sex ratio from 1:1 was seen in either the mature or immature germ cell population. The spatial distribution of X and Y chromosome-specific signals in the sperm head were identical. The hybridization treatments did not have any preferential effect on the cells of specific genotype (X or Y). Neither head parameters (length, HL; width, HW; area, HA) nor tail length (TL) significantly differed between X and Y populations of spermatozoa under any of the treatments. Similarly, the haploid, X-specific round cells did not differ from Y-specific ones by their size (diameter) and shape. These results indicate that neither mature sperm nor their precursors possess significant morphological differences between X and Y genotypes.

AB - Human X and Y spermatozoa were previously compared by several nonmolecular techniques. Recent studies show that in many of the previous investigations, the methods used to identify the spermatozoa were nonspecific and thus produced contradictory findings. In the present study, the comparison of the 2 germ cell types, X and Y, were performed following fluorescence in situ hybridization (FISH), which is the most reliable genotyping technique currently available. The FISH technique was performed under 3 different treatments: permeabilization with liquid N2, fixation with Carnoy's, and chromatin decondensation with lithium di-iodosalicylate. Mature and immature germ cells (spermatozoa and spermatids) were compared. Lithium showed higher hybridization efficiency, while liquid N2 and Carnoy's fixative maintained better morphological integrity of cells with lower hybridization. The sperm exhibiting hybridization signals were not different in any of the morphometric or qualitative comparisons from those that did not exhibit signals. No significant deviation of the sex ratio from 1:1 was seen in either the mature or immature germ cell population. The spatial distribution of X and Y chromosome-specific signals in the sperm head were identical. The hybridization treatments did not have any preferential effect on the cells of specific genotype (X or Y). Neither head parameters (length, HL; width, HW; area, HA) nor tail length (TL) significantly differed between X and Y populations of spermatozoa under any of the treatments. Similarly, the haploid, X-specific round cells did not differ from Y-specific ones by their size (diameter) and shape. These results indicate that neither mature sperm nor their precursors possess significant morphological differences between X and Y genotypes.

KW - FISH technique

KW - Morphological comparison

KW - Spermatid

UR - http://www.scopus.com/inward/record.url?scp=0035178812&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035178812&partnerID=8YFLogxK

M3 - Article

C2 - 11191075

AN - SCOPUS:0035178812

VL - 22

SP - 119

EP - 123

JO - Journal of Andrology

JF - Journal of Andrology

SN - 0196-3635

IS - 1

ER -