Lack of Th17 cell generation in patients with severe burn injuries

Akihito Inatsu, Mari Kogiso, Marc G. Jeschke, Akira Asai, Makiko Kobayashi, David N. Herndon, Fujio Suzuki

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

Immunodeficient patients with severe burn injuries are extremely susceptible to infection with Candida albicans. In addition to Th1 cells, IL-17-producing CD4+ T cells (Th17 cells) have recently been described as an important effector cell in host anti-Candida resistance. In this study, therefore, we tried to induce Th17 cells in cultures of severely burned patient PBMC by stimulation with the C. albicans Ag (CAg). In the results, the biomarkers for Th17 cells (IL-17 production and intracellular expression of IL-17 and retinoic acid receptor-related orphan receptor gt) were not displayed by burn patient PBMC stimulated with CAg, whereas these biomarkers of Th17 cells were detected in cultures of healthy donor PBMC stimulated with CAg. Burn patient sera were shown to be inhibitory on CAg-stimulated Th17 cell generation in healthy donor PBMC cultures; however, Th17 cells were induced by CAg in healthy donor PBMC cultures supplemented with burn patient sera that were previously treated with anti-IL-10 mAb. Also, the biomarkers of Th17 cells were not induced by CAg in healthy donor PBMC cultures supplemented with rIL-10. IL-10 was detected in serum specimens derived from severely burned patients. These results indicate that Th17 cells are not generated in burn patient PBMC cultures supplemented with CAg. IL-10, produced in response to burn injuries, is shown to be inhibitory on Th17 cell generation. The high susceptibility of severely burned patients to C. albicans infection might be influenced if burn-associated IL-10 production is intervened.

Original languageEnglish (US)
Pages (from-to)2155-2161
Number of pages7
JournalJournal of Immunology
Volume187
Issue number5
DOIs
StatePublished - Sep 1 2011

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Fingerprint Dive into the research topics of 'Lack of Th17 cell generation in patients with severe burn injuries'. Together they form a unique fingerprint.

  • Cite this

    Inatsu, A., Kogiso, M., Jeschke, M. G., Asai, A., Kobayashi, M., Herndon, D. N., & Suzuki, F. (2011). Lack of Th17 cell generation in patients with severe burn injuries. Journal of Immunology, 187(5), 2155-2161. https://doi.org/10.4049/jimmunol.1003235