Lack of uncoupling of S phase and mitosis after irradiation in p53- human lymphoblast cell lines

Frederik Wenz, Yongjia Yu, Hatsumi Nagasawa, Amy C. Imrich, Peter Keng, John B. Little

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

It has been shown that p53- human colorectal cancer cells arrest after DNA damage in a G2-like state and may then undergo DNA synthesis without intervening mitosis (Waldman et al., Nature 381, 713-716, 1996). To further clarify the role of p53 in the regulation of the G2/M-phase checkpoint, we have studied cells of three closely related human lymphoblastoid cell lines (TK6, WTK1 and TK6E6, an HPV16 E6-transfected TK6 line) with differing p53 status. The cells were irradiated with 1.5-12 Gy γ rays with or without 2 mM caffeine. There was no evidence of uncoupling of DNA synthesis and mitosis after irradiation in the p53-cell lines, WTK1 and TK6E6, suggesting that this uncoupling may not be a universal phenomenon. The apparent formation of tetraploid cells after irradiation of cells of the p53- WTK1 line was due to the occurrence of a G2-phase block in a pre-existing tetraploid population. These results support the conclusion that control of the G2/M-phase checkpoint after irradiation may differ among different cell types.

Original languageEnglish (US)
Pages (from-to)129-134
Number of pages6
JournalRadiation Research
Volume148
Issue number2
DOIs
StatePublished - Aug 1997
Externally publishedYes

Fingerprint

mitosis
S Phase
cultured cells
Mitosis
interphase
irradiation
cell lines
Cell Line
deoxyribonucleic acid
cells
G2 Phase Cell Cycle Checkpoints
M Phase Cell Cycle Checkpoints
Tetraploidy
tetraploidy
caffeine
synthesis
human cell lines
DNA
rays
colorectal neoplasms

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Radiology Nuclear Medicine and imaging
  • Biophysics
  • Radiation

Cite this

Lack of uncoupling of S phase and mitosis after irradiation in p53- human lymphoblast cell lines. / Wenz, Frederik; Yu, Yongjia; Nagasawa, Hatsumi; Imrich, Amy C.; Keng, Peter; Little, John B.

In: Radiation Research, Vol. 148, No. 2, 08.1997, p. 129-134.

Research output: Contribution to journalArticle

Wenz, Frederik ; Yu, Yongjia ; Nagasawa, Hatsumi ; Imrich, Amy C. ; Keng, Peter ; Little, John B. / Lack of uncoupling of S phase and mitosis after irradiation in p53- human lymphoblast cell lines. In: Radiation Research. 1997 ; Vol. 148, No. 2. pp. 129-134.
@article{06445aad3d4341bc9466f86f94f61f92,
title = "Lack of uncoupling of S phase and mitosis after irradiation in p53- human lymphoblast cell lines",
abstract = "It has been shown that p53- human colorectal cancer cells arrest after DNA damage in a G2-like state and may then undergo DNA synthesis without intervening mitosis (Waldman et al., Nature 381, 713-716, 1996). To further clarify the role of p53 in the regulation of the G2/M-phase checkpoint, we have studied cells of three closely related human lymphoblastoid cell lines (TK6, WTK1 and TK6E6, an HPV16 E6-transfected TK6 line) with differing p53 status. The cells were irradiated with 1.5-12 Gy γ rays with or without 2 mM caffeine. There was no evidence of uncoupling of DNA synthesis and mitosis after irradiation in the p53-cell lines, WTK1 and TK6E6, suggesting that this uncoupling may not be a universal phenomenon. The apparent formation of tetraploid cells after irradiation of cells of the p53- WTK1 line was due to the occurrence of a G2-phase block in a pre-existing tetraploid population. These results support the conclusion that control of the G2/M-phase checkpoint after irradiation may differ among different cell types.",
author = "Frederik Wenz and Yongjia Yu and Hatsumi Nagasawa and Imrich, {Amy C.} and Peter Keng and Little, {John B.}",
year = "1997",
month = "8",
doi = "10.2307/3579569",
language = "English (US)",
volume = "148",
pages = "129--134",
journal = "Radiation Research",
issn = "0033-7587",
publisher = "Radiation Research Society",
number = "2",

}

TY - JOUR

T1 - Lack of uncoupling of S phase and mitosis after irradiation in p53- human lymphoblast cell lines

AU - Wenz, Frederik

AU - Yu, Yongjia

AU - Nagasawa, Hatsumi

AU - Imrich, Amy C.

AU - Keng, Peter

AU - Little, John B.

PY - 1997/8

Y1 - 1997/8

N2 - It has been shown that p53- human colorectal cancer cells arrest after DNA damage in a G2-like state and may then undergo DNA synthesis without intervening mitosis (Waldman et al., Nature 381, 713-716, 1996). To further clarify the role of p53 in the regulation of the G2/M-phase checkpoint, we have studied cells of three closely related human lymphoblastoid cell lines (TK6, WTK1 and TK6E6, an HPV16 E6-transfected TK6 line) with differing p53 status. The cells were irradiated with 1.5-12 Gy γ rays with or without 2 mM caffeine. There was no evidence of uncoupling of DNA synthesis and mitosis after irradiation in the p53-cell lines, WTK1 and TK6E6, suggesting that this uncoupling may not be a universal phenomenon. The apparent formation of tetraploid cells after irradiation of cells of the p53- WTK1 line was due to the occurrence of a G2-phase block in a pre-existing tetraploid population. These results support the conclusion that control of the G2/M-phase checkpoint after irradiation may differ among different cell types.

AB - It has been shown that p53- human colorectal cancer cells arrest after DNA damage in a G2-like state and may then undergo DNA synthesis without intervening mitosis (Waldman et al., Nature 381, 713-716, 1996). To further clarify the role of p53 in the regulation of the G2/M-phase checkpoint, we have studied cells of three closely related human lymphoblastoid cell lines (TK6, WTK1 and TK6E6, an HPV16 E6-transfected TK6 line) with differing p53 status. The cells were irradiated with 1.5-12 Gy γ rays with or without 2 mM caffeine. There was no evidence of uncoupling of DNA synthesis and mitosis after irradiation in the p53-cell lines, WTK1 and TK6E6, suggesting that this uncoupling may not be a universal phenomenon. The apparent formation of tetraploid cells after irradiation of cells of the p53- WTK1 line was due to the occurrence of a G2-phase block in a pre-existing tetraploid population. These results support the conclusion that control of the G2/M-phase checkpoint after irradiation may differ among different cell types.

UR - http://www.scopus.com/inward/record.url?scp=0030852882&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030852882&partnerID=8YFLogxK

U2 - 10.2307/3579569

DO - 10.2307/3579569

M3 - Article

C2 - 9254731

AN - SCOPUS:0030852882

VL - 148

SP - 129

EP - 134

JO - Radiation Research

JF - Radiation Research

SN - 0033-7587

IS - 2

ER -