TY - JOUR
T1 - Lactate dehydrogenase activity in mouse lung following 1,1-dichloroethylene
T2 - Index of airway injury
AU - Forkert, P. G.
AU - Custer, E. M.
AU - Alpert, A. J.
AU - Ansari, G. A.S.
AU - Reynolds, E. S.
PY - 1982
Y1 - 1982
N2 - Lactate dehydrogenase (LD) activity and its isozyme profile in mouse lung homogenate was affected by oral administration of 1,1-dichloroethylene (1,1-DCE). Following 100 mg 1,1-DCElkg, LD-3 increased significantly. After 200 mg 1,1-DCE1kg, LD-5 increased whereas LD-1 and LD-2 decreased, with a resultant higher M:H ratio than controls. In contrast, elevated LD activity in serum following 1,1-DCE was predominately associated with striking increases in total activity and changes in isozyme patterns resulting in a decrease in the M:H ratio. LD activity in liver and erythrocytes were unaffected by 1,1, -DCE administration. Although total activity in kidney was decreased, no changes were detected in the isozyme profile. Pulmonary damage induced by 1,1 -DCE was reflected in significant increases in total activity and all isozymes in bronchopulmonary lavage fluids. Thus, detection of lung-derived LD activity in lung lavage fluids can be a useful index of pulmonary airway injury.
AB - Lactate dehydrogenase (LD) activity and its isozyme profile in mouse lung homogenate was affected by oral administration of 1,1-dichloroethylene (1,1-DCE). Following 100 mg 1,1-DCElkg, LD-3 increased significantly. After 200 mg 1,1-DCE1kg, LD-5 increased whereas LD-1 and LD-2 decreased, with a resultant higher M:H ratio than controls. In contrast, elevated LD activity in serum following 1,1-DCE was predominately associated with striking increases in total activity and changes in isozyme patterns resulting in a decrease in the M:H ratio. LD activity in liver and erythrocytes were unaffected by 1,1, -DCE administration. Although total activity in kidney was decreased, no changes were detected in the isozyme profile. Pulmonary damage induced by 1,1 -DCE was reflected in significant increases in total activity and all isozymes in bronchopulmonary lavage fluids. Thus, detection of lung-derived LD activity in lung lavage fluids can be a useful index of pulmonary airway injury.
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U2 - 10.3109/01902148209039250
DO - 10.3109/01902148209039250
M3 - Article
C2 - 7169062
AN - SCOPUS:0020262173
SN - 0190-2148
VL - 4
SP - 67
EP - 77
JO - Experimental Lung Research
JF - Experimental Lung Research
IS - 1
ER -