Lactate dehydrogenase activity in mouse lung following 1,1-dichloroethylene

Index of airway injury

P. G. Forkert, E. M. Custer, A. J. Alpert, Ghulam Ansari, E. S. Reynolds

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Lactate dehydrogenase (LD) activity and its isozyme profile in mouse lung homogenate was affected by oral administration of 1,1-dichloroethylene (1,1-DCE). Following 100 mg 1,1-DCElkg, LD-3 increased significantly. After 200 mg 1,1-DCE1kg, LD-5 increased whereas LD-1 and LD-2 decreased, with a resultant higher M:H ratio than controls. In contrast, elevated LD activity in serum following 1,1-DCE was predominately associated with striking increases in total activity and changes in isozyme patterns resulting in a decrease in the M:H ratio. LD activity in liver and erythrocytes were unaffected by 1,1, -DCE administration. Although total activity in kidney was decreased, no changes were detected in the isozyme profile. Pulmonary damage induced by 1,1 -DCE was reflected in significant increases in total activity and all isozymes in bronchopulmonary lavage fluids. Thus, detection of lung-derived LD activity in lung lavage fluids can be a useful index of pulmonary airway injury.

Original languageEnglish (US)
Pages (from-to)67-77
Number of pages11
JournalExperimental Lung Research
Volume4
Issue number1
DOIs
StatePublished - 1982

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L-Lactate Dehydrogenase
Isoenzymes
Lung
Wounds and Injuries
Fluids
Bronchoalveolar Lavage Fluid
Lung Injury
Bronchoalveolar Lavage
Liver
Oral Administration
Erythrocytes
Kidney
vinylidene chloride
Serum

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Molecular Biology
  • Clinical Biochemistry

Cite this

Lactate dehydrogenase activity in mouse lung following 1,1-dichloroethylene : Index of airway injury. / Forkert, P. G.; Custer, E. M.; Alpert, A. J.; Ansari, Ghulam; Reynolds, E. S.

In: Experimental Lung Research, Vol. 4, No. 1, 1982, p. 67-77.

Research output: Contribution to journalArticle

Forkert, P. G. ; Custer, E. M. ; Alpert, A. J. ; Ansari, Ghulam ; Reynolds, E. S. / Lactate dehydrogenase activity in mouse lung following 1,1-dichloroethylene : Index of airway injury. In: Experimental Lung Research. 1982 ; Vol. 4, No. 1. pp. 67-77.
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