Lactate production in isolated segments of the rat nephron.

S. Bagnasco, David Good, R. Balaban, M. Burg

Research output: Contribution to journalArticle

108 Citations (Scopus)

Abstract

Lactate production was measured directly in individual segments of the rat nephron. Tubules were dissected and then incubated in vitro with glucose as the only metabolic substrate. Each segment was incubated with and without antimycin A, an inhibitor of oxidative metabolism. Proximal tubules produced no lactate with or without antimycin A. The distal segments all produced lactate. The rate of lactate production without antimycin A ranged from 0.4 to 0.9 pmol X min-1 X mm-1 in all distal segments except one, the inner medullary collecting duct, which produced lactate at the significantly higher rate of 2.8 pmol X min-1 X mm-1. Antimycin A increased lactate production significantly in all of the distal segments. The increase was largest in medullary thick ascending limbs (1,400%) and cortical (798%) and outer medullary collecting ducts (357%). Increments were smaller in cortical thick ascending limbs (98%) and distal convoluted tubules (98%) and least in the inner medullary collecting ducts (28%). We conclude that lactate production occurs only in distal segments of the nephron and that under anoxic conditions significant amounts of ATP are produced by anaerobic glycolysis in these segments.

Original languageEnglish (US)
JournalThe American journal of physiology
Volume248
Issue number4 Pt 2
StatePublished - Apr 1985
Externally publishedYes

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Nephrons
Lactic Acid
Antimycin A
Extremities
Glycolysis
Adenosine Triphosphate
Glucose

ASJC Scopus subject areas

  • Medicine(all)

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Lactate production in isolated segments of the rat nephron. / Bagnasco, S.; Good, David; Balaban, R.; Burg, M.

In: The American journal of physiology, Vol. 248, No. 4 Pt 2, 04.1985.

Research output: Contribution to journalArticle

Bagnasco, S. ; Good, David ; Balaban, R. ; Burg, M. / Lactate production in isolated segments of the rat nephron. In: The American journal of physiology. 1985 ; Vol. 248, No. 4 Pt 2.
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