Laminin interactions with head and neck cancer cells under low fluid shear conditions lead to integrin activation and binding

Susan M. Fennewald, Carla Kantara, Sarita K. Sastry, Vicente Resto

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Lymphatic metastasis of cancer cells involves movement from the primary tumor site to the lymph node, where the cells must be able to productively lodge and grow. It is there that tumor cells encounter cellular and non-cellular constituent elements that make up the lymph node parenchyma. Our work shows that head and neck squamous cell carcinoma (HNSCC) cell lines are able to bind to laminin, fibronectin, vitronectin, and hyaluronic acid, which are extracellular matrix elements within the lymph node parenchyma. HNSCC cell lines bound to laminin under lymphodynamic low shear stress (0.07 dynes/cm 2), consistent with lymph flow via β1 integrins, including α2β1, α3β1, and α6β1. Binding occurred in the presence of shear stress and not in the absence of flow. Additionally, tumor cell binding to laminin under flow did result in calcium signaling. Our data indicate a novel role for β1 integrin-mediated binding of HNSCC cells to laminin under conditions of lymphodynamic flow that results in intracellular calcium signaling within the cancer cell.

Original languageEnglish (US)
Pages (from-to)21058-21066
Number of pages9
JournalJournal of Biological Chemistry
Volume287
Issue number25
DOIs
StatePublished - Jun 15 2012

Fingerprint

Laminin
Head and Neck Neoplasms
Integrins
Chemical activation
Cells
Fluids
Calcium Signaling
Lymph Nodes
Tumors
Neoplasms
Shear stress
Lymphatic Metastasis
Vitronectin
Cell Line
Calcium
Lymph
Hyaluronic Acid
Fibronectins
Cell Movement
Extracellular Matrix

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Laminin interactions with head and neck cancer cells under low fluid shear conditions lead to integrin activation and binding. / Fennewald, Susan M.; Kantara, Carla; Sastry, Sarita K.; Resto, Vicente.

In: Journal of Biological Chemistry, Vol. 287, No. 25, 15.06.2012, p. 21058-21066.

Research output: Contribution to journalArticle

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