Abstract
Eastern equine encephalitis virus (EEEV) has a high case-fatality rate in horses and humans, and Florida has been hypothesized to be the source of EEEV epidemics for the northeastern United States. To test this hypothesis, we sequenced complete genomes of 433 EEEV strains collected within the United States from 1934 to 2014. Phylogenetic analysis suggested EEEV evolves relatively slowly and that transmission is enzootic in Florida, characterized by higher genetic diversity and long-term local persistence. In contrast, EEEV strains in New York and Massachusetts were characterized by lower genetic diversity, multiple introductions, and shorter local persistence. Our phylogeographic analysis supported a source-sink model in which Florida is the major source of EEEV compared to the other localities sampled. In sum, this study revealed the complex epidemiological dynamics of EEEV in different geographic regions in the United States and provided general insights into the evolution and transmission of other avian mosquito-borne viruses in this region.
| Original language | English (US) |
|---|---|
| Article number | e00074-18 |
| Journal | Journal of Virology |
| Volume | 92 |
| Issue number | 12 |
| DOIs | |
| State | Published - Jun 1 2018 |
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Keywords
- EEEV
- Evolution
- Next-generation sequencing
- NGS
- Phylodynamics
- Phylogeography
- Source-sink dynamics
ASJC Scopus subject areas
- Microbiology
- Immunology
- Insect Science
- Virology
Cite this
Large-scale complete-genome sequencing and phylodynamic analysis of eastern equine encephalitis virus reveals source-sink transmission dynamics in the United States. / Tan, Yi; Lam, Tommy Tsan Yuk; Heberlein-Larson, Lea A.; Smole, Sandra C.; Auguste, Albert J.; Hennigan, Scott; Halpin, Rebecca A.; Fedorova, Nadia; Puri, Vinita; Stockwell, Timothy B.; Shilts, Meghan H.; Andreadis, Theodore; Armstrong, Philip M.; Tesh, Robert B.; Weaver, Scott; Unnasch, Thomas R.; Ciota, Alexander T.; Kramer, Laura D.; Das, Suman R.
In: Journal of Virology, Vol. 92, No. 12, e00074-18, 01.06.2018.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Large-scale complete-genome sequencing and phylodynamic analysis of eastern equine encephalitis virus reveals source-sink transmission dynamics in the United States
AU - Tan, Yi
AU - Lam, Tommy Tsan Yuk
AU - Heberlein-Larson, Lea A.
AU - Smole, Sandra C.
AU - Auguste, Albert J.
AU - Hennigan, Scott
AU - Halpin, Rebecca A.
AU - Fedorova, Nadia
AU - Puri, Vinita
AU - Stockwell, Timothy B.
AU - Shilts, Meghan H.
AU - Andreadis, Theodore
AU - Armstrong, Philip M.
AU - Tesh, Robert B.
AU - Weaver, Scott
AU - Unnasch, Thomas R.
AU - Ciota, Alexander T.
AU - Kramer, Laura D.
AU - Das, Suman R.
PY - 2018/6/1
Y1 - 2018/6/1
N2 - Eastern equine encephalitis virus (EEEV) has a high case-fatality rate in horses and humans, and Florida has been hypothesized to be the source of EEEV epidemics for the northeastern United States. To test this hypothesis, we sequenced complete genomes of 433 EEEV strains collected within the United States from 1934 to 2014. Phylogenetic analysis suggested EEEV evolves relatively slowly and that transmission is enzootic in Florida, characterized by higher genetic diversity and long-term local persistence. In contrast, EEEV strains in New York and Massachusetts were characterized by lower genetic diversity, multiple introductions, and shorter local persistence. Our phylogeographic analysis supported a source-sink model in which Florida is the major source of EEEV compared to the other localities sampled. In sum, this study revealed the complex epidemiological dynamics of EEEV in different geographic regions in the United States and provided general insights into the evolution and transmission of other avian mosquito-borne viruses in this region.
AB - Eastern equine encephalitis virus (EEEV) has a high case-fatality rate in horses and humans, and Florida has been hypothesized to be the source of EEEV epidemics for the northeastern United States. To test this hypothesis, we sequenced complete genomes of 433 EEEV strains collected within the United States from 1934 to 2014. Phylogenetic analysis suggested EEEV evolves relatively slowly and that transmission is enzootic in Florida, characterized by higher genetic diversity and long-term local persistence. In contrast, EEEV strains in New York and Massachusetts were characterized by lower genetic diversity, multiple introductions, and shorter local persistence. Our phylogeographic analysis supported a source-sink model in which Florida is the major source of EEEV compared to the other localities sampled. In sum, this study revealed the complex epidemiological dynamics of EEEV in different geographic regions in the United States and provided general insights into the evolution and transmission of other avian mosquito-borne viruses in this region.
KW - EEEV
KW - Evolution
KW - Next-generation sequencing
KW - NGS
KW - Phylodynamics
KW - Phylogeography
KW - Source-sink dynamics
UR - http://www.scopus.com/inward/record.url?scp=85047662565&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85047662565&partnerID=8YFLogxK
U2 - 10.1128/JVI.00074-18
DO - 10.1128/JVI.00074-18
M3 - Article
C2 - 29618651
AN - SCOPUS:85047662565
VL - 92
JO - Journal of Virology
JF - Journal of Virology
SN - 0022-538X
IS - 12
M1 - e00074-18
ER -