@article{ad6d2b9a9e71410fbcae432be5a6303d,
title = "Large-scale complete-genome sequencing and phylodynamic analysis of eastern equine encephalitis virus reveals source-sink transmission dynamics in the United States",
abstract = "Eastern equine encephalitis virus (EEEV) has a high case-fatality rate in horses and humans, and Florida has been hypothesized to be the source of EEEV epidemics for the northeastern United States. To test this hypothesis, we sequenced complete genomes of 433 EEEV strains collected within the United States from 1934 to 2014. Phylogenetic analysis suggested EEEV evolves relatively slowly and that transmission is enzootic in Florida, characterized by higher genetic diversity and long-term local persistence. In contrast, EEEV strains in New York and Massachusetts were characterized by lower genetic diversity, multiple introductions, and shorter local persistence. Our phylogeographic analysis supported a source-sink model in which Florida is the major source of EEEV compared to the other localities sampled. In sum, this study revealed the complex epidemiological dynamics of EEEV in different geographic regions in the United States and provided general insights into the evolution and transmission of other avian mosquito-borne viruses in this region.",
keywords = "EEEV, Evolution, NGS, Next-generation sequencing, Phylodynamics, Phylogeography, Source-sink dynamics",
author = "Yi Tan and Lam, {Tommy Tsan Yuk} and Heberlein-Larson, {Lea A.} and Smole, {Sandra C.} and Auguste, {Albert J.} and Scott Hennigan and Halpin, {Rebecca A.} and Nadia Fedorova and Vinita Puri and Stockwell, {Timothy B.} and Shilts, {Meghan H.} and Theodore Andreadis and Armstrong, {Philip M.} and Tesh, {Robert B.} and Weaver, {Scott C.} and Unnasch, {Thomas R.} and Ciota, {Alexander T.} and Kramer, {Laura D.} and Das, {Suman R.}",
note = "Funding Information: This project has been funded in whole or in part with federal funds from the National Institute of Allergy and Infectious Diseases, National Institutes of Health, Department of Health and Human Services, under award U19AI110819. This study was also supported by a grant from the National Institutes of Health to T.R.U. (grant R56AI01372). This publication was supported by Cooperative Agreement no. NU50CK000423, funded by the Centers for Disease Control and Prevention. S.R.D. is also supported by the NIH-funded Tennessee Center for AIDS Research (P30 AI110527). The content is solely the responsibility of the authors and does not represent official views of the Centers for Disease Control and Prevention, the National Institutes of Health, the Florida State Department of Health, the Massachusetts Department of Public Health, or the New York State Department of Health, USA.",
year = "2018",
month = jun,
day = "1",
doi = "10.1128/JVI.00074-18",
language = "English (US)",
volume = "92",
journal = "Journal of Virology",
issn = "0022-538X",
publisher = "American Society for Microbiology",
number = "12",
}