Laser capture microdissection in traumatic brain injury research: Obtaining hippocampal subregions and pools of injured neurons for genomic analyses

Deborah R. Boone, Harris A. Weisz, Stacy Sell, Helen Hellmich

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The methods presented here are based on our laboratory’s 15 years of experience using laser capture microdissection to obtain samples for the study of gene expression after traumatic brain injury (TBI) using a well-established rat model of experimental TBI. Here, we describe how to use the ArcturusXT laser capture microdissection system to capture swaths of specific regions of the rat hippocampus as well as specific neuronal populations defined by Fluoro-Jade C staining. Staining with Fluoro-Jade C identifies a neuron that is in the process of degeneration. We have optimized our protocols for Fluoro-Jade C tissue staining and laser capture microdissection to maintain RNA integrity which is essential for a variety of downstream applications, such as microarray, PCR array, and quantitative real-time PCR analyses.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages235-245
Number of pages11
Volume1723
DOIs
StatePublished - Jan 1 2018

Publication series

NameMethods in Molecular Biology
Volume1723
ISSN (Print)1064-3745

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Keywords

  • Brain injury
  • Fluoro-Jade C
  • Gene expression
  • Hippocampus
  • Neuron
  • Single cell

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Boone, D. R., Weisz, H. A., Sell, S., & Hellmich, H. (2018). Laser capture microdissection in traumatic brain injury research: Obtaining hippocampal subregions and pools of injured neurons for genomic analyses. In Methods in Molecular Biology (Vol. 1723, pp. 235-245). (Methods in Molecular Biology; Vol. 1723). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7558-7_13