Laser Doppler velocimetry of tracheal blood flow in sheep

Vernon W. Lin, George Kramer, Gibbe H. Parsons, Carroll E. Cross

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A laser-Doppler velocimetry (LDV) apparatus was adapted to assess sheep airway blood flow. The LDV signal obtained was compared to microsphere determinations of blood flow to tracheal tissues utilizing 15 μm radioactive microspheres injected before and after intubation and anesthesia, during hemorrhagic hypotension, and after reinfusion-resuscitation. During hemorrhagic hypotension, airway wall blood flow decreased to 16% of control by the microsphere method and to 30% by LDV. After reinfusion-resuscitation, airway wall blood flow increased over control values 40% and 33% by the two methods, respectively. Although at low flows LDV values were greater than microsphere determinations, the overall LDV recordings correlated with airway microsphere flow determinations of tracheal wall blood flow (R = 0.85) and tracheal mucosa flow (R = 0.81), but not with tracheal muscularis flow (R = 0.32). With certain but significant limitations, such as calibration in absolute units, stability of position placement, motion artifacts, and the effects of mechanical irritation-induced hyperemia, LDV represents a relatively noninvasive means for qualitatively evaluating changes in the microcirculatory blood flow of airway mucosa.

Original languageEnglish (US)
Pages (from-to)341-354
Number of pages14
JournalRespiration Physiology
Volume85
Issue number3
DOIs
StatePublished - 1991
Externally publishedYes

Fingerprint

Laser-Doppler Flowmetry
Sheep
Microspheres
Resuscitation
Hypotension
Mucous Membrane
Hyperemia
Intubation
Artifacts
Calibration
Anesthesia

Keywords

  • Airways, blood flow
  • Blood flow, airways
  • Laser Doppler velocimetry, comparison with microsphere injection
  • Mammal, sheep

ASJC Scopus subject areas

  • Physiology
  • Pulmonary and Respiratory Medicine

Cite this

Laser Doppler velocimetry of tracheal blood flow in sheep. / Lin, Vernon W.; Kramer, George; Parsons, Gibbe H.; Cross, Carroll E.

In: Respiration Physiology, Vol. 85, No. 3, 1991, p. 341-354.

Research output: Contribution to journalArticle

Lin, Vernon W. ; Kramer, George ; Parsons, Gibbe H. ; Cross, Carroll E. / Laser Doppler velocimetry of tracheal blood flow in sheep. In: Respiration Physiology. 1991 ; Vol. 85, No. 3. pp. 341-354.
@article{947452fd6f4d4c78b00a90e49bec8ae6,
title = "Laser Doppler velocimetry of tracheal blood flow in sheep",
abstract = "A laser-Doppler velocimetry (LDV) apparatus was adapted to assess sheep airway blood flow. The LDV signal obtained was compared to microsphere determinations of blood flow to tracheal tissues utilizing 15 μm radioactive microspheres injected before and after intubation and anesthesia, during hemorrhagic hypotension, and after reinfusion-resuscitation. During hemorrhagic hypotension, airway wall blood flow decreased to 16{\%} of control by the microsphere method and to 30{\%} by LDV. After reinfusion-resuscitation, airway wall blood flow increased over control values 40{\%} and 33{\%} by the two methods, respectively. Although at low flows LDV values were greater than microsphere determinations, the overall LDV recordings correlated with airway microsphere flow determinations of tracheal wall blood flow (R = 0.85) and tracheal mucosa flow (R = 0.81), but not with tracheal muscularis flow (R = 0.32). With certain but significant limitations, such as calibration in absolute units, stability of position placement, motion artifacts, and the effects of mechanical irritation-induced hyperemia, LDV represents a relatively noninvasive means for qualitatively evaluating changes in the microcirculatory blood flow of airway mucosa.",
keywords = "Airways, blood flow, Blood flow, airways, Laser Doppler velocimetry, comparison with microsphere injection, Mammal, sheep",
author = "Lin, {Vernon W.} and George Kramer and Parsons, {Gibbe H.} and Cross, {Carroll E.}",
year = "1991",
doi = "10.1016/0034-5687(91)90073-R",
language = "English (US)",
volume = "85",
pages = "341--354",
journal = "Respiratory Physiology and Neurobiology",
issn = "1569-9048",
publisher = "Elsevier",
number = "3",

}

TY - JOUR

T1 - Laser Doppler velocimetry of tracheal blood flow in sheep

AU - Lin, Vernon W.

AU - Kramer, George

AU - Parsons, Gibbe H.

AU - Cross, Carroll E.

PY - 1991

Y1 - 1991

N2 - A laser-Doppler velocimetry (LDV) apparatus was adapted to assess sheep airway blood flow. The LDV signal obtained was compared to microsphere determinations of blood flow to tracheal tissues utilizing 15 μm radioactive microspheres injected before and after intubation and anesthesia, during hemorrhagic hypotension, and after reinfusion-resuscitation. During hemorrhagic hypotension, airway wall blood flow decreased to 16% of control by the microsphere method and to 30% by LDV. After reinfusion-resuscitation, airway wall blood flow increased over control values 40% and 33% by the two methods, respectively. Although at low flows LDV values were greater than microsphere determinations, the overall LDV recordings correlated with airway microsphere flow determinations of tracheal wall blood flow (R = 0.85) and tracheal mucosa flow (R = 0.81), but not with tracheal muscularis flow (R = 0.32). With certain but significant limitations, such as calibration in absolute units, stability of position placement, motion artifacts, and the effects of mechanical irritation-induced hyperemia, LDV represents a relatively noninvasive means for qualitatively evaluating changes in the microcirculatory blood flow of airway mucosa.

AB - A laser-Doppler velocimetry (LDV) apparatus was adapted to assess sheep airway blood flow. The LDV signal obtained was compared to microsphere determinations of blood flow to tracheal tissues utilizing 15 μm radioactive microspheres injected before and after intubation and anesthesia, during hemorrhagic hypotension, and after reinfusion-resuscitation. During hemorrhagic hypotension, airway wall blood flow decreased to 16% of control by the microsphere method and to 30% by LDV. After reinfusion-resuscitation, airway wall blood flow increased over control values 40% and 33% by the two methods, respectively. Although at low flows LDV values were greater than microsphere determinations, the overall LDV recordings correlated with airway microsphere flow determinations of tracheal wall blood flow (R = 0.85) and tracheal mucosa flow (R = 0.81), but not with tracheal muscularis flow (R = 0.32). With certain but significant limitations, such as calibration in absolute units, stability of position placement, motion artifacts, and the effects of mechanical irritation-induced hyperemia, LDV represents a relatively noninvasive means for qualitatively evaluating changes in the microcirculatory blood flow of airway mucosa.

KW - Airways, blood flow

KW - Blood flow, airways

KW - Laser Doppler velocimetry, comparison with microsphere injection

KW - Mammal, sheep

UR - http://www.scopus.com/inward/record.url?scp=0025769658&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025769658&partnerID=8YFLogxK

U2 - 10.1016/0034-5687(91)90073-R

DO - 10.1016/0034-5687(91)90073-R

M3 - Article

VL - 85

SP - 341

EP - 354

JO - Respiratory Physiology and Neurobiology

JF - Respiratory Physiology and Neurobiology

SN - 1569-9048

IS - 3

ER -