Laser induced resonance energy transfer - A novel approach towards achieving high sensitivity in capillary electrophoresis Part B: Applications for environmental testing

M. G. Bissell, A. O. Okorodudu, J. R. Petersen, Amin A. Mohammad

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Capillary electrophoresis with laser induced resonance energy transfer detection is used for direct determination of ethylenediamine tetraacetic acid (EDTA), 1,2-diaminopropane-N,N,N',N'-tetraacetic acid (DPTA) and (+)-trans-1,2-diaminocyclohexane-N,N,N',N'-tetraacetic acid (DCTA) in aqueous samples. The method involves CZE separation of salicylate-Tb-EDTA, salicylate-Tb-DPTA and salicylate-Tb-DCTA ternary complexes at alkaline pH. The highly efficient transfer of energy from salicylate to terbium in these complexes facilitated a sensitive detection. Detection limits in the 1 · 10-7 M range are easily achieved. A 325 nm HeCd laser is used for excitation and terbium luminescence is monitored at 547 nm. The potential application of this method in analyzing these acids in a complex radioactive waste is discussed.

Original languageEnglish (US)
Pages (from-to)217-221
Number of pages5
JournalJournal of Chromatography A
Volume767
Issue number1-2
DOIs
StatePublished - Apr 11 1997

Keywords

  • 1,2-Diaminopropane-N,N,N',N'-tetraacetic acid
  • Detection, electrophoresis
  • Ethylenediaminetetraacetic acid
  • Laser-induced resonance energy transfer
  • Resonance energy transfer
  • trans-1,2-Diaminocyclohexane-N,N,N',N'-tetraacetic acid

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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