Laser induced resonance energy transfer - A novel approach towards achieving high sensitivity in capillary electrophoresis I. Clinical diagnostic application

J. R. Petersen, M. G. Bissell, Amin A. Mohammad

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

Most of the procedures currently performed by capillary zone electrophoresis (CZE) with laser induced fluorescence detection requires prior derivatization. This increases cost, the turn-around-time and chances of extraneous contaminations. CZE with laser induced resonance energy transfer is demonstrated as a viable alternative for detecting non-fluorescent compounds with no prior derivatization. The feasibility of this approach is demonstrated by separating and directly detecting salicylic acid (2,4-dihydroxybenzoic acid), gentisic acid (o-methoxybenzoic acid), salicyluric acid (o-hydroxyhippuric acid) and 4-aminosalicylic acid in urine. The detection of salicylate in serum is also shown. The method is highly sensitive with detection limits in the 1 · 10-7 M range. Importantly it requires no prior preconcentration or sample preparation and can be used with complex sample matrices such as serum and urine.

Original languageEnglish (US)
Pages (from-to)37-44
Number of pages8
JournalJournal of Chromatography A
Volume744
Issue number1-2
DOIs
StatePublished - Sep 13 1996

Keywords

  • Aminosalicylic acid
  • Detection, electrophoresis
  • Genetic acid
  • Laser-induced resonance energy transfer
  • Salicylic acid
  • Salicyluric acid

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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