Laser induced resonance energy transfer - A novel approach towards achieving high sensitivity in capillary electrophoresis I. Clinical diagnostic application

J. R. Petersen, M. G. Bissell, Amin A. Mohammad

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

Most of the procedures currently performed by capillary zone electrophoresis (CZE) with laser induced fluorescence detection requires prior derivatization. This increases cost, the turn-around-time and chances of extraneous contaminations. CZE with laser induced resonance energy transfer is demonstrated as a viable alternative for detecting non-fluorescent compounds with no prior derivatization. The feasibility of this approach is demonstrated by separating and directly detecting salicylic acid (2,4-dihydroxybenzoic acid), gentisic acid (o-methoxybenzoic acid), salicyluric acid (o-hydroxyhippuric acid) and 4-aminosalicylic acid in urine. The detection of salicylate in serum is also shown. The method is highly sensitive with detection limits in the 1 · 10-7 M range. Importantly it requires no prior preconcentration or sample preparation and can be used with complex sample matrices such as serum and urine.

Original languageEnglish (US)
Pages (from-to)37-44
Number of pages8
JournalJournal of Chromatography A
Volume744
Issue number1-2
DOIs
StatePublished - Sep 13 1996

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Keywords

  • Aminosalicylic acid
  • Detection, electrophoresis
  • Genetic acid
  • Laser-induced resonance energy transfer
  • Salicylic acid
  • Salicyluric acid

ASJC Scopus subject areas

  • Analytical Chemistry

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