Laser injury and in vivo multimodal imaging using a mouse model

Ginger M. Pocock, Adam Boretsky, Praveena Gupta, Jeff W. Oliver, Massoud Motamedi

Research output: Chapter in Book/Report/Conference proceedingConference contribution


Balb/c wild type mice were used to perform in vivo experiments of laser-induced thermal damage to the retina. A Heidelberg Spectralis HRA confocal scanning laser ophthalmoscope with a spectral domain optical coherence tomographer was used to obtain fundus and cross-sectional images of laser induced injury in the retina. Sub-threshold, threshold, and supra-threshold lesions were observed using optical coherence tomography (OCT), infrared reflectance, red-free reflectance, fluorescence angiography, and autofluorescence imaging modalities at different time points post-exposure. Lesions observed using all imaging modalities, except autofluorescence, were not visible immediately after exposure but did resolve within an hour and grew in size over a 24 hour period. There was a decrease in fundus autofluorescence at exposure sites immediately following exposure that developed into hyper-fluorescence 24-48 hours later. OCT images revealed threshold damage that was localized to the RPE but extended into the neural retina over a 24 hour period. Volumetric representations of the mouse retina were created to visualize the extent of damage within the retina over a 24 hour period. Multimodal imaging provides complementary information regarding damage mechanisms that may be used to quantify the extent of the damage as well as the effectiveness of treatments without need for histology.

Original languageEnglish (US)
Title of host publicationOptical Interactions with Tissue and Cells XXII
StatePublished - 2011
EventOptical Interactions with Tissue and Cells XXII - San Francisco, CA, United States
Duration: Jan 24 2011Jan 26 2011

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


OtherOptical Interactions with Tissue and Cells XXII
Country/TerritoryUnited States
CitySan Francisco, CA


  • Confocal scanning laser ophthalmoscope
  • Laser
  • Laser threshold
  • Mouse
  • Retina
  • Retinal lesion
  • Spectral domain optical coherence tomography

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Radiology Nuclear Medicine and imaging


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