Laser injury and in vivo multimodal imaging using a mouse model

Ginger M. Pocock, Adam Boretsky, Praveena Gupta, Jeff W. Oliver, Massoud Motamedi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Balb/c wild type mice were used to perform in vivo experiments of laser-induced thermal damage to the retina. A Heidelberg Spectralis HRA confocal scanning laser ophthalmoscope with a spectral domain optical coherence tomographer was used to obtain fundus and cross-sectional images of laser induced injury in the retina. Sub-threshold, threshold, and supra-threshold lesions were observed using optical coherence tomography (OCT), infrared reflectance, red-free reflectance, fluorescence angiography, and autofluorescence imaging modalities at different time points post-exposure. Lesions observed using all imaging modalities, except autofluorescence, were not visible immediately after exposure but did resolve within an hour and grew in size over a 24 hour period. There was a decrease in fundus autofluorescence at exposure sites immediately following exposure that developed into hyper-fluorescence 24-48 hours later. OCT images revealed threshold damage that was localized to the RPE but extended into the neural retina over a 24 hour period. Volumetric representations of the mouse retina were created to visualize the extent of damage within the retina over a 24 hour period. Multimodal imaging provides complementary information regarding damage mechanisms that may be used to quantify the extent of the damage as well as the effectiveness of treatments without need for histology.

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7897
DOIs
StatePublished - 2011
EventOptical Interactions with Tissue and Cells XXII - San Francisco, CA, United States
Duration: Jan 24 2011Jan 26 2011

Other

OtherOptical Interactions with Tissue and Cells XXII
CountryUnited States
CitySan Francisco, CA
Period1/24/111/26/11

Fingerprint

Multimodal Imaging
retina
mice
Retina
Lasers
Optical tomography
Imaging techniques
Wounds and Injuries
damage
Fluorescence
Optical Imaging
lasers
Optical Coherence Tomography
Histology
Angiography
lesions
thresholds
tomography
Ophthalmoscopes
reflectance

Keywords

  • Confocal scanning laser ophthalmoscope
  • Laser
  • Laser threshold
  • Mouse
  • Retina
  • Retinal lesion
  • Spectral domain optical coherence tomography

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Pocock, G. M., Boretsky, A., Gupta, P., Oliver, J. W., & Motamedi, M. (2011). Laser injury and in vivo multimodal imaging using a mouse model. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 7897). [789713] https://doi.org/10.1117/12.873979

Laser injury and in vivo multimodal imaging using a mouse model. / Pocock, Ginger M.; Boretsky, Adam; Gupta, Praveena; Oliver, Jeff W.; Motamedi, Massoud.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7897 2011. 789713.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Pocock, GM, Boretsky, A, Gupta, P, Oliver, JW & Motamedi, M 2011, Laser injury and in vivo multimodal imaging using a mouse model. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 7897, 789713, Optical Interactions with Tissue and Cells XXII, San Francisco, CA, United States, 1/24/11. https://doi.org/10.1117/12.873979
Pocock GM, Boretsky A, Gupta P, Oliver JW, Motamedi M. Laser injury and in vivo multimodal imaging using a mouse model. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7897. 2011. 789713 https://doi.org/10.1117/12.873979
Pocock, Ginger M. ; Boretsky, Adam ; Gupta, Praveena ; Oliver, Jeff W. ; Motamedi, Massoud. / Laser injury and in vivo multimodal imaging using a mouse model. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7897 2011.
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