Lateral mobility of integral membrane proteins is increased in spherocytic erythrocytes

Michael Sheetz, Melvin Schindler, Dennis E. Koppel

Research output: Contribution to journalArticle

196 Citations (Scopus)

Abstract

Alterations of glycoprotein distribution and lateral mobility in cell membranes can provide transmembrane signals for several membrane-related phenomena1-3. Control of the transmembranous events has been ascribed to interaction between submembranous protein matrices (or 'cytoskeletons') and membrane glycoproteins4-7. A consequence of such interaction would be differential inhibition of protein lateral diffusion in biological membranes. Measurements of the lateral diffusion coefficients of membrane proteins, in fact, have generally yielded values8-12 much less than were predicted for unhindered diffusion in a fluid bilayer13,14. The mouse spherocytic erythrocyte, which lacks the major components of the normal erythrocyte membrane matrix15 (composed of spectrin, actin, bands 4.1 and 4.9 (ref. 16), in the nomenclature of Fairbanks et al.17), provides a unique system for a direct evaluation of the effect of the matrix on protein lateral mobility. After using a modification of the technique of fluorescence redistribution after photobleaching (FRAP)18, we report here that membrane proteins diffuse about 50 times faster in spherocytic than in normal mouse erythrocytes.

Original languageEnglish (US)
Pages (from-to)510-512
Number of pages3
JournalNature
Volume285
Issue number5765
DOIs
StatePublished - Dec 1 1980
Externally publishedYes

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Membrane Proteins
Erythrocytes
Membranes
Photobleaching
Spectrin
Proteins
Erythrocyte Membrane
Cytoskeleton
Terminology
Actins
Glycoproteins
Fluorescence
Cell Membrane

ASJC Scopus subject areas

  • General

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Lateral mobility of integral membrane proteins is increased in spherocytic erythrocytes. / Sheetz, Michael; Schindler, Melvin; Koppel, Dennis E.

In: Nature, Vol. 285, No. 5765, 01.12.1980, p. 510-512.

Research output: Contribution to journalArticle

Sheetz, Michael ; Schindler, Melvin ; Koppel, Dennis E. / Lateral mobility of integral membrane proteins is increased in spherocytic erythrocytes. In: Nature. 1980 ; Vol. 285, No. 5765. pp. 510-512.
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